OPTIMIZATION OF BACTERIAL LYOPHILIZATION PROCESS: INFLUENCE OF CULTIVATION AND LYOPHILIZATION PARAMETERS ON PROBIOTIC SURVIVAL RATE
Abstract
Background. The preservation of bacterial cultures plays an important role in scientific research and industrial production, both food and medical. The study of the influence of lyophilization process on bacterial survival is important for the development of effective methods of probiotic application. Based on the analysis of more than three hundred publications by domestic and foreign research groups, about thirty key factors influencing the survival of bacteria during the lyophilization process have been identified. However, for the equation describing the survival rate of bacteria during lyophilization, seventeen parameters were taken into account, which are the most significant for ensuring successful lyophilization of bacterial cultures. It is worth noting that when considered in detail, many factors in the overall survival equation are impossible or very difficult to trace. Therefore, in the presented work, conditions have been taken to simplify the equation. Identification of the value function reflecting how valuable the influence of the introduced parameter on the survival of microorganisms after cryofreezing will allow to create a virtual process model for the study of bacterial survival.
Purpose. The purpose of this review is to summarize and discuss the parameters affecting bacterial survival during lyophilization. Obtaining a value function is important for optimizing the process and improving the preservation efficiency of microorganisms. This paper discusses the role of various influencing factors on bacterial survival rate during multistage lyophilization and their impact on the quality of preserved probiotics.
Materials and methods. A literature search was performed using the following electronic databases: Google Scholar, Science Direct, PudMed, and CyberLeninka. Search queries included combinations of the following phrases: "bacterial lyophilization", "probiotic research", "cryopreservation", "bacterial survival equation for lyophilization", "value function".
Results. Bacterial cultures are an important component of many scientific and medical studies. Preservation of bacterial cultures is important to ensure their viability and usefulness for future research. There are several methods of preserving bacterial cultures including refrigeration, freezing and lyophilization. This paper presents the factors affecting bacterial mass accumulation during cultivation and the factors affecting bacterial survival rate during lyophilization. Based on the selected parameters, an equation including the most significant parameters affecting bacterial survival rate during cryopreservation and lyophilization is presented. However, a detailed examination of the equation revealed parameters that are constant in most studies when dealing with lactic acid bacteria. This paper presents an overview of the parameters and modes of cultivation and lyophilization that affect the survival rate of bacteria. Various factors such as preparation steps, cultivation parameters and freezing affect the bacterial survival function during lyophilization. Understanding these factors is key to optimize the lyophilization process and improve microbial preservation efficiency. Further research in this area will help to improve the quality of preserved probiotics and broaden the scope of their application.
Conclusion. Thus, with an increase in infectious load or atrophic changes in the mucosa of the stomach does not exclude the exhaustion of reserve capacity of phagocytes, their modification, thus the transformation of precancerous condition in cancer.
EDN: YNAEBU
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Copyright (c) 2024 Mariia S. Ashikhmina, Vladislav S. Filozop, Mikhail O. Volodarskiy, Sviatlana A. Ulasevich, Ekaterina V. Skorb
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