Composition and predicted functions of dairy cattle's endometrial and rumen microbiome against the background of chronic endometritis
Abstract
Background. Scientific basis. Endometritis is one of the most important causes of dairy cattle maleness, leading to significant economic losses in animal husbandry. The composition of the microbiome of cattle has a direct impact on their reproductive health.
Purpose. Assess study purposes was to evaluate changes in the composition and predicted metabolic pathways of the endometrial and rumen microbiota of cattle with chronic endometritis.
Materials and methods. For the experiment two groups of cows were formed during the milking period (n = 3): control group I - clinically healthy, as well as with good reproduction indicators in the anamnesis of life, experimental group II - with chronic endometritis. The composition of the microbial community of endometrial scraping and rumen chyme was assessed using targeted NGS sequencing. Reconstruction and prediction of the functional content of gene and enzyme families were carried out using the PICRUSt2 software package (v.2.3.0).
Results. In the rumen of cattle of experimental group II, compared with control I, a decrease in the number of Ruminococcus sp. was noted and Veillonella sp. by 2.0 and 2.8 times, respectively, against the background of an increase in Fusobacterium sp. and bacteria of the order Bacteroidales – genera Bacteroides and Porphyromonas by 1,7-2,6 times (P<0.05).
A sharp (by 8.6%) increase in the number of Fusobacterium sp. (P<0.05) was noted in the endometrium of cattle of experimental group II compared with control I. In the endometrial microbiota, against the background of the disease, there was complete suppression of important pathways associated with the synthesis of vitamins, such as menaquinol-10 and biotin, as well as coenzyme Q10 (ubiquinols-7-10) associated with antioxidant activity compared to control group I (P<0.05).
Conclusion. Our findings indicate that chronic endometritis in cattle appear as a mixed infection, the etiological origin of which, in possibility, is an increase in the number of microorganisms present in the reproductive system of clinically healthy cows. The results of our study provide additional information about the mechanisms of development of chronic endometritis in cattle.
EDN: QWWMIL
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Copyright (c) 2025 Elena A. Yildirim, Valentina A. Filippova, Larisa A. Ilina, Georgy Yu. Laptev, Andrey V. Dubrovin, Vasily A. Zaikin, Ksenia A. Kalitkina, Elena A. Korochkina, Irina A. Klyuchnikova, Ekaterina S. Ponomareva, Evgeny A. Brazhnik, Tatyana S. Smetannikova, Daria G. Tyurina
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