Race-specific leaf and root resistance of winter oilseed rape (Brassica napus L.) to Xanthomonas campestris pv. campestris

Anastasiia V. Vishnyakova; Mikhail A. Nikitin; Olesya O. Rumiantseva; Aleksei A. Mironov; Sokrat G. Monakhos

doi:10.12731/2658-6649-2025-17-2-1043

Anastasiia V. Vishnyakova Russian State Agrarian University - Moscow Timiryazev Agricultural Academy https://orcid.org/0000-0002-9160-1164
Mikhail A. Nikitin Selection and Seed Center for Vegetable Crops Russian State Agrarian University - Timiryazev Moscow Agricultural Academy https://orcid.org/0009-0007-5557-1192
Olesya O. Rumiantseva Russian State Agrarian University - Moscow Timiryazev Agricultural Academy https://orcid.org/0009-0002-3248-7514
Aleksei A. Mironov Russian State Agrarian University - Moscow Timiryazev Agricultural Academy https://orcid.org/0000-0002-0297-500X
Sokrat G. Monakhos Russian State Agrarian University - Moscow Timiryazev Agricultural Academy https://orcid.org/0000-0001-9404-8862

DOI: https://doi.org/10.12731/2658-6649-2025-17-2-1043

Keywords: winter oilseed rape, Xanthomonas campestris pv. campestris (Xcc), root resistance, leaf resistance, races, disease resistance

Abstract

Background. Researchers from France, Serbia, and Russia have reported the spread of Black Rot in winter rape crop. The cultivation of resistant varieties and hybrids is considered to be the most effective method of reducing the economic damage caused by the disease. The complexity of breeding for resistance to Black Rot lies in the presence of at least 11 races of the pathogen Xanthomonas campestris pv. campestris, each of which requires its own resistance gene. Studies of race-specific resistance of winter rapeseed are limited to the study of resistance to 1 and 4 races of Xanthomonas campestris pv. campestris using leaf-piercing technique.

Purpose. The objective of this research is to study the manifestation of leaf and root resistance of rapeseed to Black Rot and to identify sources of high race-specific resistance to 1, 3, 4, and 6 races of Xanthomonas campestris pv. campestris.

Materials and methods. Artificial screening of resistance to black rot was carried out using a genetic collection of winter rapeseed, represented by 30 accessions. Inoculation using bacterial suspensions of Xanthomonas campestris pv. сampestris was conducted under controlled conditions of a climate chamber. The inoculation process involved leaf-piercing technique by clipping the leaf edges near the veins using forceps or root traumatization. Black rot resistance or susceptibility were estimated on a two-point scale.

Results. One source of leaf resistance to race 4 of Xanthomonas campestris pv. campestris “Tegg” was identified in the genetic collection of winter rapseed. When plants were inoculated using the root traumatization system, the samples Dagg with root resistance to Xanthomonas campestris pv. campestris race 4 and Fagg with root resistance to race 3 were identified.

Conclusion. Two independent race-specific systems of resistance to black rot have been identified in winter rapeseed, which are manifested using different methods of inoculation with Xanthomonas campestris pv. campestris pathogen. The identified sources of resistance can be used in rapeseed breeding programs for resistance. Hybridologic and molecular genetic analysis of each of the identified resistance systems is required.

EDN: KKBEWM

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Author Biographies

Anastasiia V. Vishnyakova, Russian State Agrarian University - Moscow Timiryazev Agricultural Academy

Cand. Sc. (Agricultural), Associate Professor of the Department of Botany, Selection and Seed Production of Garden Plants

Mikhail A. Nikitin, Selection and Seed Center for Vegetable Crops Russian State Agrarian University - Timiryazev Moscow Agricultural Academy

research engineer

Olesya O. Rumiantseva, Russian State Agrarian University - Moscow Timiryazev Agricultural Academy

1st year Master Student of the Department of Botany, Selection and Seed Production of Garden Plants

Aleksei A. Mironov, Russian State Agrarian University - Moscow Timiryazev Agricultural Academy

Cand. Sc. (Agricultural), Assistant professor, Associate Professor of the Department of Botany, Selection and Seed Production of Garden Plants

Sokrat G. Monakhos, Russian State Agrarian University - Moscow Timiryazev Agricultural Academy

Dr. Sc. (Agricultural), Professor, Head of the Head of the Department of Botany, Selection and Seed Production of Garden Plants

References

Артемьева, А. М., Игнатов, А. Н., Волкова, А. И., Кочерина, Н. В., Коноплева, М. Н., & Чесноков, Ю. В. (2018). Физиолого-генетические компоненты устойчивости к сосудистому бактериозу у линий удвоенных гаплоидов Brassica rapa L. Сельскохозяйственная биология, 53(1), 157-169. https://doi.org/10.15389/agrobiology.2018.1.157rus EDN: https://elibrary.ru/vzwyix

Во Тхи Нгок Ха, Джалилов, Ф. С., Виноградова, С. В., Кырова, Е. И., & Игнатов, А. Н. (2014). Генетическое разнообразие возбудителя сосудистого бактериоза в России: полиморфизм пцр фрагментов. Защита картофеля, (2), 21-25.

Во Тхи Нгок Ха, Джалилов, Ф. С., & Игнатов, А. Н. (2015). Оценка устойчивости различных гибридов белокочанной капусты к сосудистому бактериозу. Вестник Российского университета дружбы народов. Серия: Агрономия и животноводство, (2), 7-15.

Во Тхи Нгок Ха, Джалилов, Ф. С., Мазурин, Е. С., Кырова, Е. И., Виноградова, С. В., Шаад, Н. В., Ластер, Д., & Игнатов, А. Н. (2014). Распространение нового генотипа Xanthomonas campestris pv. campestris в России в 2012 гг. Защита картофеля, (2), 26-28.

Джалилов, Ф. С., Корсак, И. В., & Монахос, Г. Ф. (1995). Сравнение методов оценки устойчивости капусты к сосудистому бактериозу. Известия Тимирязевской сельскохозяйственной академии, (2), 147-153.

Игнатов, А. Н., Джалилов, Ф. С., Мирошников, К. А., & Евсеев, П. В. (2021). Xanthomonas campestris: основные результаты и проблемы исследования возбудителя сосудистого бактериоза капустных. Микробные биотехнологии: фундаментальные и прикладные аспекты, 13, 153-168. https://doi.org/10.47612/2226-3136-2021-13-153-168 EDN: https://elibrary.ru/qpqqt

Лазарев, А. М., Мысник, Е. Н., & Игнатов, А. Н. (2017). Ареал и зона вредоносности сосудистого бактериоза капусты. Вестник защиты растений, 1(91), 52-55.

Матвеева, Е. В., Игнатов, А. Н., Политыко, В. А., & Фокина, В. Г. (2008). Бактериальные болезни рапса. Защита и карантин растений, (12), 23-24.

Монахос, С. Г., & Елышко, Н. В. (2015). Устойчивость капусты к сосудистому бактериозу. Картофель и овощи, (9), 38-39.

Орынбаев, А. Т., Джалилов, Ф. С., & Монахос, Г. Ф. (2019). Методы оценки и характер наследования стеблевой устойчивости к сосудистому бактериозу у белокочанной капусты. Известия Тимирязевской сельскохозяйственной академии, (1), 45-55.

Afrin, K. S., Rahim, M. A., Rubel, M. H., Natarajan, S., Song, J., Kim, H., Park, J., & Nou, I. (2018). Development of race-specific molecular marker for Xanthomonas campestris pv. campestris race 3, the causal agent of black rot of crucifers. Canadian Journal of Plant Science, 98(5), 1119-1125. https://doi.org/10.1139/cjps-2018-0035

Ignatov, A., Kuginuki, Y., & Hida, K. (1998). Race-specific reaction of resistance to black rot in Brassica oleracea. European Journal of Plant Pathology, 104(8), 821-827. https://doi.org/10.1023/A:1008642829156

Ignatov, A., Kuginuki, Y., & Hida, K. (2000). Distribution and inheritance of race-specific resistance to Xanthomonas campestris pv. campestris in Brassica rapa and B. napus. Journal of Russian Phytopathology, 1, 89-94.

Jelušić, A., Berić, T., Mitrović, P., Dimkić, I., Stanković, S., Marjanović-Jeromela, A., & Popović, T. (2020). New insights into the genetic diversity of Xanthomonas campestris pv. campestris isolates from winter oilseed rape in Serbia. Plant Pathology, 70(1), 35-49. https://doi.org/10.1111/ppa.13273 EDN: https://elibrary.ru/cpiysj

Lema, M., Soengas, P., Velasco, P., Francisco, M., & Cartea, M. E. (2011). Identification of Sources of Resistance to Xanthomonas campestris pv. campestris in Brassica napus Crops. Plant Disease, 95(3), 292-297. https://doi.org/10.1094/PDIS-06-10-0428 EDN: https://elibrary.ru/okxkzr

Lu, L., Monakhos, S. G., Lim, Y. P., & Yi, S. Y. (2021). Early defense mechanisms of Brassica oleracea in response to attack by Xanthomonas campestris pv. campestris. Plants, 10(12), 2705. https://doi.org/10.3390/plants10122705 EDN: https://elibrary.ru/kvcjgg

Mansfield, J., Genin, S., Magori, S., Citovsky, V., Sriariyanum, M., Ronald, P., Dow, M., Verdier, V., Beer, S. V., Machado, M. A., Toth, I., Salmond, G., & Foster, G. D. (2012). Top 10 plant pathogenic bacteria in molecular plant pathology. Molecular Plant Pathology, 13(6), 614-629. https://doi.org/10.1111/j.1364-3703.2012.00804.x EDN: https://elibrary.ru/rntqyz

Popović, T., Balaž, J., Starović, M., Trkulja, N., Ivanović, Ž., Ignjatov, M., & Jošić, D. (2013). First Report of Xanthomonas campestris pv. campestris as the Causal Agent of Black Rot on Oilseed Rape (Brassica napus) in Serbia. Plant Disease, 97(3), 418. https://doi.org/10.1094/PDIS-07-22-1389-PDN

Popović, T., Mitrović, P., Jelušić, A., Dimkić, I., Marjanović-Jeromela, A., Nikolić, I., & Stanković, S. (2019). Genetic diversity and virulence of Xanthomonas campestris pv. campestris isolates from Brassica napus and six Brassica oleracea crops in Serbia. Plant Pathology, 68(8), 1448-1457. https://doi.org/10.1111/ppa.13064 EDN: https://elibrary.ru/vnoukw

Rubel, M. H., Robin, A. H. K., Natarajan, S., Vicente, J. G., Kim, H., Park, J., & Nou, I. (2017). Whole-Genome Re-Alignment Facilitates Development of Specific Molecular Markers for Races 1 and 4 of Xanthomonas campestris pv. campestris, the Cause of Black Rot Disease in Brassica oleracea. International Journal of Molecular Sciences, 18(12), 2523. https://doi.org/10.3390/ijms18122523 EDN: https://elibrary.ru/ykarre

Vicente, J. G., Taylor, J. D., Sharpe, A. G., Parkin, I. A. P., Lydiate, D. J., & King, G. J. (2002). Inheritance of race-specific resistance to Xanthomonas campestris pv. campestris in Brassica Genomes. Phytopathology, 92(10), 1032-1148. https://doi.org/10.1094/PHYTO.2002.92.10.113 EDN: https://elibrary.ru/lzvbmj

Vicente, J. G., & Holub, E. B. (2012). Xanthomonas campestris pv. campestris (cause of black rot of crucifers) in the genomic era is still a worldwide threat to brassica crops. Molecular Plant Pathology, 14(1), 2-18. https://doi.org/10.1111/j.1364-3703.2012.00833.x EDN: https://elibrary.ru/rouenv

Zubko, O., Monakhos, S., & Monakhos, G. (2018). Rb gene introgression from Brassica carinata to Brassica oleracea. Acta Hortic, 1202, 107-112. https://doi.org/10.17660/ActaHortic.2018.1202.16 EDN: https://elibrary.ru/aeuuxk

References

Artemyeva, A. M., Ignatov, A. N., Volkova, A. I., Kocherina, N. V., Konopleva, M. N., & Chesnokov, Yu. V. (2018). Physiological and genetic components of resistance to black rot in doubled haploid lines of Brassica rapa L. Agricultural Biology, 53(1), 157-169. https://doi.org/10.15389/agrobiology.2018.1.157rus EDN: https://elibrary.ru/vzwyix

Vo Thi Ngoc Ha, Dzhalilov, F. S., Vinogradova, S. V., Kirovа, E. I., & Ignatov, A. N. (2014). Genetic diversity of the black rot pathogen in Russia: PCR fragment polymorphism. Potato Protection, (2), 21-25.

Vo Thi Ngoc Ha, Dzhalilov, F. S., & Ignatov, A. N. (2015). Evaluation of resistance of different white cabbage hybrids to black rot. Bulletin of the Peoples' Friendship University of Russia. Series: Agronomy and Animal Husbandry, (2), 7-15.

Vo Thi Ngoc Ha, Dzhalilov, F. S., Mazurin, E. S., Kirovа, E. I., Vinogradova, S. V., Shaad, N. V., Laster, D., & Ignatov, A. N. (2014). Spread of a new genotype of Xanthomonas campestris pv. campestris in Russia in 2012. Potato Protection, (2), 26-28.

Dzhalilov, F. S., Korsak, I. V., & Monakhos, G. F. (1995). Comparison of methods for assessing the resistance of cabbage to black rot. Izvestiya Timiryazevskaya Agricultural Academy, (2), 147-153.

Ignatov, A. N., Dzhalilov, F. S., Miroshnikov, K. A., & Evseev, P. V. (2021). Xanthomonas campestris: main results and problems of research of the black rot pathogen of cabbage. Microbial Biotechnology: Fundamental and Applied Aspects, 13, 153-168. https://doi.org/10.47612/2226-3136-2021-13-153-168 EDN: https://elibrary.ru/qpqqt

Lazarev, A. M., Mysnik, E. N., & Ignatov, A. N. (2017). Range and damage zone of cabbage black rot. Plant Protection Bulletin, 1(91), 52-55.

Matveeva, E. V., Ignatov, A. N., Polityko, V. A., & Fokina, V. G. (2008). Bacterial diseases of rapeseed. Protection and Quarantine of Plants, (12), 23-24.

Monakhos, S. G., & Elyshko, N. V. (2015). Resistance of cabbage to black rot. Potato and Vegetables, (9), 38-39.

Orynbaev, A. T., Dzhalilov, F. S., & Monakhos, G. F. (2019). Methods of assessment and inheritance of stem resistance to black rot in white cabbage. Izvestiya Timiryazevskaya Agricultural Academy, (1), 45-55.