INVESTIGATION OF NEUTROPHILS UNDER TECHNOLOGICAL STRESS IN COWS BY METHODS OF LASER INTERFERENCE AND ELECTRON SCANNING MICROSCOPY
Abstract
Background. Intensive milk production technology creates a number of factors that can put an animal into a state of technological stress. The effect of stress is reflected in nonspecific resistance, which represents the body's ability to resist the effects of adverse factors by maintaining protective forces at the proper level. Neutrophils are one of the first protective cellular barriers that are rapidly mobilized when exposed to stress factors. To decipher the changes taking place in response to the action of stressors, the involvement of various evaluation tools is required. One of the possible methodological approaches is the use of various visualization methods that provide access to spatio-temporal information at the level of individual organelles and
Purpose. The study of the morpho-functional state of neutrophils under technological stress in cattle by laser interference and electron scanning microscopy.
Materials and methods. The work was carried out on 30 clinically healthy highly productive black-and-white cows. A combination of impacts was considered as technological stress: the rearrangement of animals and the change of service personnel. Neutrophils were analyzed by laser interference and electron scanning microscopy.
Results. A change in the morphological structure and functional activity of neutrophils after the action of technological stress is shown. The analysis of interferograms revealed an increase in the heterogeneity of neutrophils due to the appearance of degeneratively altered cell forms with an increase in the number of functionally active neutrophils. Electron scanning microscopy revealed the appearance of NEToses and neutrophils with emptied granules and vacuolization of the cytoplasm after technological stress.
Conclusion. The revealed morphofunctional changes in neutrophils prove the strain of adaptation mechanisms under technological stress. This circumstance must be taken into account in order to prevent disruption of the adaptive capabilities of the organism of highly productive animals.
Downloads
References
Список литературы
Андрюков Б.Г. и соавт. Антимикробные стратегии нейтрофилов при инфекционной патологии / Б.Г. Андрюков, Л.М. Сомова, Е.И. Дробот, Е.В. Матосова // Клиническая лабораторная диагностика. 2016. Т. 61. №12. С. 825.
Василенко И.А. и др. Цитометрия нейтрофилов в оценке эффективности комплексного лечения больных остеомиелитом нижней челюсти / И.А. Василенко, А.А. Никитин, Н.В. Малыченко, И.А. Иванюта, В.Б. Метелин, Б.Я. Агаджанян // Альманах клинической медицины. 2008. №18. С. 64.
Гаврилова Г.А. и др. Неспецифическая резистентность коров, инфицированных ВЛКРС и больных лейкозом / Г.А. Гаврилова, С.В. Бахметьева // Сибирский вестник сельскохозяйственной науки. 2005. №1. С. 118.
Демидчик Л.А. Исследование нейтрофилов у больных с экзогенными токсическими нефропатиями / Л.А. Демидчик, Е.В. Клочкова, Е.А. Рассохина // Современные проблемы науки и образования. 2018. №6. С. 49.
Дерюгина А.В. и др. Трансляционные изменеия электрофоретической подвижности и фазового портрета эритроцитов с учетом развития стрессовой реакции в условиях патологического процесса / А.В. Дерюгина, М.Н. Иващенко, П.С. Игнатьев, А.Г. Самоделкин // Альманах клинической медицины. 2018. Т. 46. №8. С. 765-771.
Дерюгина А.В. и др. Применение лазерной интерференционной микроскопии для оценки функционального состояния эритроцитов / А.В. Дерюгина, М.Н. Иващенко, А.А. Белов, П.С. Игнатьев, В.Б. Метелин // Цитология. 2021. Т. 63. №1. С. 74.
Загубиженко М.В. и др. Использование метода лазерной интерференционной микроскопии для исследования состояния перитонеальных макрофагов мыши, облученных ультрафиолетовым светом / Загубиженко М.В., Юсипович А.И., Пирутин С.К., Минаев В.Л., Кудряшов Ю.Б. // Радиационная биология. Радиоэкология. 2011. Т. 51. №6. С. 715.
Красильникова В.Л. и др. Сканирующая электронная микроскопия в изучении поверхностных тканевых реакций при выборе титановых имплантатов для реконструктивной хирургии области орбиты / Загубиженко М.В., Юсипович А.И., Пирутин С.К., Минаев В.Л., Кудряшов Ю.Б. // Инновационные технологии в медицине. 2018. №1. С. 36.
Пискарев И.М., Иванова И.П., Самоделкин А.Г., Иващенко М.Н.. Инициирование и исследование свободно-радикальных процессов в биологических экспериментах. Нижний Новгород. 2016. 106 с.
Тузлуков И.И. Сравнительная оценка морфологических и иммунологических методик определения активности нейтрофилов / И.И. Тузлуков // Российский медико-биологический вестник имени академика И. П. Павлова. 2004. №3-4. С.113.
Тычинский В.П. Динамическая фазовая микроскопия: возможен ли «диалог» с клеткой? / В.П. Тычинский // Успехи физических наук. 2007. Т. 177. №5. С. 535.
Тюмина Н.А. и др. Низковакуумная сканирующая электронная микроскопия тучных клеток на поверхности надпочечника крысы / Н.А. Тюмина, К.Г. Кемоклидзе, Д.Э. Пухов // Морфология. 2016. Т. 149. №3 С. 211.
Castanheira F.V.S. Neutrophils and NETS in modulating acute and chronic inflammation // Blood. 2019. №133. Р. 2178-2185. https://doi.org/10.1182/blood-2018-11-844530
D’Cruz A.A. The pseudokinase MLKL activates PAD4-dependent NET formation in necroptotic neutrophils / A.A. D’Cruz, M. Speir, M. Bliss-Moreau, S. Dietrich, S. Wang // Sci. Signal. 2018. Vol. 11. https://doi.org/10.1126/scisignal.aao1716
Desai J. PMA and crystal-induced neutrophil extracellular trap formation involves RIPK1-RIPK3-MLKL signaling / J. Desai, S.V. Kumar, S.R. Mulay, L. Konrad, S. Romoli // Eur. J. Immunol. 2016. №46. Р. 223. https://doi.org/10.1002/eji.201545605
G..E. Helal Effect of noise stress and/or sulpiride treatment on some physiological and histological parameters in female albino rats / G..E. Helal, F. Eid, M.T. Neama // The Egypt J. hospital med. 2011. V. 44. P. 295
Kesner E.E. Characteristics of mitochondrial transformation into human cells / E.E. Kesner, A. Saada-Reich, H. Lorberboum-Galski // Sci. Rep. 2016. №6. Р. 26057. https://doi.org/10.1038/srep26057
Mantovani A. Neutrophils in the activation and regulation of innate and adaptive immunity / A. Mantovani, M.A. Cassatella, C. Costantini, S. Jaillon // Nat. Rev. Immunol. 2011. Vol. 11. Р. 519. https://doi.org/10.1038/nri3024
Moschonas I.C. The pathway of neutrophil extracellular traps towards atherosclerosis and thrombosis / I.C. Moschonas, A.D. Tselepis // Atherosclerosis. 2019. V. 288. P. 9. https://doi.org/10.1016/j.atherosclerosis.2019.06.919
Reeves E.R. Killing activity of neutrophils is mediated through activation of proteases by K+ flux / E.R. Reeves, H. Lu, R.T. Jacobs // Nature. 2002. Vol. 416 (6878). Р. 291. https://doi.org/10.1038/416291a
Romero L.M. Physiological stress in ecology: lessons from biomedical research // Trends in Ecology and Evolution. 2004. Vol. 19. Р. 249. https://doi.org/10.1016/j.tree.2004.03.008
Spees J.L. Mitochondrial transfer between cells can rescue aerobic respiration / J.L. Spees, S.D. Olson, M.J. Whitney, D.J. Prockop // Proc.Natl.Acad. Sci. U.S.A. 2006. Vol. 103. Р. 1283. https://doi.org/10.1073/pnas.0510511103
Yipp B.G. Infection-induced NETosis is a dynamic process involving neutrophil multitasking in vivo / B.G. Yipp, B. Petri, D. Salina// Nature medicine. 2012. V. 18. P. 1386. https://doi.org/10.1038/nm.2847
References
Andriukov B.G. et al. Antimikrobnye strategii nejtrofilov pri infekcionnoj patologii [Antimicrobial strategies of neutrophils in infectious pathology] / B.G. Andriukov, L.M. Somova, E.I. Drobot, E.V. Matosova. Klinicheskaya laboratornaya diagnostika [Clinical laboratory diagnostics], 2016, vol. 61, no. 12, p. 825.
Vasilenko I.A. et al. Citometriya nejtrofilov v ocenke effektivnosti kompleksnogo lecheniya bol'nyh osteomielitom nizhnej chelyusti [Cytometry of neutrophils in assessing the effectiveness of complex treatment of patients with osteomyelitis of the lower jaw] / I.A. Vasilenko, A.A. Nikitin, N.V. Malychenko, I.A. Ivanyuta, V.B. Metelin, B.Ya. Aghajanyan. Al'manah klinicheskoj mediciny [Almanac of clinical Medicine], 2008, no. 18, p. 64.
Gavrilova G.A. et al. Nespecificheskaya rezistentnost' korov, inficirovannyh VLKRS i bol'nyh lejkozom [Nonspecific resistance of cows infected with VLKRS and patients with leukemia] / G.A. Gavrilova, S.V. Bakhmetyeva. Sibirskij vestnik sel'skohozyajstvennoj nauki [Siberian Bulletin of Agricultural Science], 2005, no. 1, p. 118.
Demidchik L.A. Issledovanie nejtrofilov u bol'nyh s ekzogennymi toksicheskimi nefropatiyami [Study of neutrophils in patients with exogenous toxic nephropathies] / L.A. Demidchik, E.V. Klochkova, E.A. Rassokhina. Sovremennye problemy nauki i obrazovaniya [Modern problems of science and education], 2018, no. 6, p. 49.
Deryugina A.V. et al. Translyacionnye izmeneiya elektroforeticheskoj podvizhnosti i fazovogo portreta eritrocitov s uchetom razvitiya stressovoj reakcii v usloviyah patologicheskogo processa [Translational changes in electrophoretic mobility and phase portrait of erythrocytes taking into account the development of a stress reaction in a pathological process] / A.V. Deryugina, M.N. Ivashchenko, P.S. Ignatiev, A.G. Samodelkin. Al'manah klinicheskoj mediciny [Almanac of Clinical Medicine], 2018, vol. 46, no. 8, pp. 765-771.
Deryugina A.V. et al. Primenenie lazernoj interferencionnoj mikroskopii dlya ocenki funkcional'nogo sostoyaniya eritrocitov [Application of laser interference microscopy to assess the functional state of erythrocytes] / A.V. Deryugina, M.N. Ivashchenko, A.A. Belov, P.S. Ignatiev, V.B. Metelin. Citologiya [Cytology], 2021, vol. 63, no. 1, p. 74.
Zagubizhenko M.V. et al. Ispol'zovanie metoda lazernoj interferencionnoj mikroskopii dlya issledovaniya sostoyaniya peritoneal'nyh makrofagov myshi, obluchennyh ul'trafioletovym svetom [Using the method of laser interference microscopy to study the state of mouse peritoneal macrophages irradiated with ultraviolet light] / M.V. Zagubizhenko, A.I. Yusipovich, S.K. Pirutin, V.L. Minaev, Yu.B. Kudryashov. Radiacionnaya biologiya. Radioekologiya. [Radiation Biology. Radioecology], 2011, vol. 51, no. 6, p. 715.
Krasilnikova V.L. et al. Skaniruyushchaya elektronnaya mikroskopiya v izuchenii poverhnostnyh tkanevyh reakcij pri vybore titanovyh implantatov dlya rekonstruktivnoj hirurgii oblasti orbity [Scanning electron microscopy in the study of surface tissue reactions when choosing titanium implants for reconstructive surgery of the orbital region] / M.V. Zagubizhenko, A.I. Yusipovich, S.K. Pirutin, V.L. Minaev, Yu.B. Kudryashov. Innovacionnye tekhnologii v medicine [Innovative technologies in medicine], 2018, no. 1, p. 36.
Piskarev I.M., Ivanova I.P., Samodelkin A.G., Ivashchenko M.N. Iniciirovanie i issledovanie svobodno-radikal'nyh processov v biologicheskih eksperimentah [Initiation and investigation of free radical processes in biological experiments]. Nizhniy Novgorod, 2016, 106 p.
Tuzlukov I.I. Sravnitel'naya ocenka morfologicheskih i immunologicheskih metodik opredeleniya aktivnosti nejtrofilov [Comparative assessment of morphological and immunological methods for determining the activity of neutrophils]. Rossijskij mediko-biologicheskij vestnik imeni akademika I.P. Pavlova [Russian Biomedical Bulletin named after Academician I.P. Pavlov], 2004, no. № 3-4, p. 113.
Tychinsky V.P. Dinamicheskaya fazovaya mikroskopiya: vozmozhen li «dialog» s kletkoj? [Dynamic phase microscopy: is a "dialogue" with a cell possible?]. Uspekhi fizicheskih nauk [Successes of physical sciences], 2007, vol. 177, no. 5, p. 535.
Tyumina N.A. et al. Nizkovakuumnaya skaniruyushchaya elektronnaya mikroskopiya tuchnyh kletok na poverhnosti nadpochechnika krysy [Low-vacuum scanning electron microscopy of mast cells on the surface of the rat adrenal gland] / N.A. Tyumina, K.G. Kemoklidze, D.E. Pukhov. Morfologiya [Morphology], 2016, vol. 149, no. 3, p. 211.
Castanheira F.V.S. Neutrophils and NETS in modulating acute and chronic inflammation. Blood., 2019, no. 133, pp. 2178-2185. https://doi.org/10.1182/blood-2018-11-844530.
D’Cruz A.A. The pseudokinase MLKL activates PAD4-dependent NET formation in necroptotic neutrophils / A.A. D’Cruz, M. Speir, M. Bliss-Moreau, S. Dietrich, S. Wang. Sci. Signal., 2018, vol. 11. https://doi.org/10.1126/scisignal.aao1716
Desai J. PMA and crystal-induced neutrophil extracellular trap formation involves RIPK1-RIPK3-MLKL signaling / J. Desai, S. V. Kumar, S.R. Mulay, L. Konrad, S. Romoli. Eur. J. Immunol., 2016, no. 46, p. 223. https://doi.org/10.1002/eji.201545605
Helal G.E. Effect of noise stress and/or sulpiride treatment on some physiological and histological parameters in female albino rats / G.E. Helal, F. Eid, M.T. Neama. The Egypt J. hospital med., 2011, vol. 44, p. 295.
Kesner E.E. Characteristics of mitochondrial transformation into human cells / E.E. Kesner, A. Saada-Reich, H. Lorberboum-Galski. Sci. Rep., 2016, no. 6, 26057. https://doi.org/10.1038/srep26057
Mantovani A. Neutrophils in the activation and regulation of innate and adaptive immunity / A. Mantovani, M.A. Cassatella, C. Costantini, S. Jaillon. Nat. Rev. Immunol., 2011, vol. 11, p. 519. https://doi.org/10.1038/nri3024
Moschonas I.C. The pathway of neutrophil extracellular traps towards atherosclerosis and thrombosis / I.C. Moschonas, A.D. Tselepis. Atherosclerosis, 2019, vol. 288, p. 9. https://doi.org/10.1016/j.atherosclerosis.2019.06.919
Reeves E.R. Killing activity of neutrophils is mediated through activation of proteases by K+ flux / E.R. Reeves, H. Lu, R.T. Jacobs. Nature, 2002, vol. 416 (6878), p. 291. https://doi.org/10.1038/416291a
Romero L.M. Physiological stress in ecology: lessons from biomedical research. Trends in Ecology and Evolution, 2004, vol. 19, p. 249. https://doi.org/10.1016/j.tree.2004.03.008
Spees J.L. Mitochondrial transfer between cells can rescue aerobic respiration / J.L. Spees, S.D. Olson, M.J. Whitney, D.J. Prockop. Proc.Natl.Acad. Sci. U.S.A., 2006, vol. 103, p. 1283. https://doi.org/10.1073/pnas.0510511103
Yipp B.G. Infection-induced NETosis is a dynamic process involving neutrophil multitasking in vivo / B.G. Yipp, B. Petri, D. Salina. Nature medicine, 2012, vol. 18, p. 1386. https://doi.org/10.1038/nm.2847
Copyright (c) 2024 Anna V. Deryugina, Marina N. Ivashchenko, Vladislav B. Metelin, Roman S. Kovylin, Pavel S. Ignatiev
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
