Genotyping of Gleditsia triacanthos L. based on RbcL gene expression and using ISSR markers in arid climate conditions
Abstract
Background. Fighting against desertification is one of the priorities in the world. Gleditsia triacanthos L. is a promising species for afforestation as it has high drought resistance. Genotyping of G. triacanthos has not been previously conducted based on the study of photosynthetic productivity by evaluating the expression of the large subunit gene of RuBisCo - RbcL and linking it to ISSR markers.
Purpose. To conduct the genotyping of individuals in the G. triacanthos population by evaluation the quantitative expression of the large subunit gene of RuBisCo - RbcL and their linkage to ISSR markers.
Materials and methods. The study was carried out at the arboretum of the FSC of agroecology RAS, where 10 individuals of G. triacanthos in good vital state were selected. The expression of RbcL was evaluated using RT-PCR. ISSR analysis was performed to examine the genetic structure of the studied G. triacanthos individuals. Statistical analysis of experimental data was performed using the software package Statistica 12.0 (StatSoft, USA) and POPGENE version 1.31.
Results. Based on the results of the RbcL expression evaluation, individuals with different transcriptional activity were identified: 20% of G. triacanthos individuals had high expression, 40% had moderate expression, and 40% had low expression. Effective primers from the UBC group, 836 and 873, were identified during ISSR analysis in G. triacanthos individuals. Genotyping by ISSR revealed two groups of G. triacanthos individuals with high and low RbcL expression.
Conclusion. The acquired data can potentially be used in molecular breeding of G. Triacanthos to obtain not only drought-resistant genotypes, but also genotypes with high photosynthetic productivity to address agroforestry tasks.
EDN: WKRCPL
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References
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