Study of the effect of gamma radiation on the antibiotic activity of osmotic microbiota in some types of urbechs

Keywords: urbech, microorganisms, osmophilic yeast, antioxidants, Mild preservatives, gamma irradiation, kGy, doses of ionizing radiation

Abstract

Background. “Urbech” – traditional national product of Dagestan peoples recently found increasing popularity among adherents of a healthy diet. Urbech made according to traditional recipes retains its properties when stored for more than 1-2 years. Nowadays urbech range is constantly expanding. Some of the new types of urbech can spoil in 5-7 days.

Materials and methods. The samples of urbech made from coconut flakes and dried mulberries spoiled most quickly, peanut urbech with grated cocoa beans and date syrup, and sesame urbech with honey. The urbech has been treated with antioxidants and mild preservatives and has been exposed to gamma radiation. Using standard methods, the authors have determined humidity, pH, acid number of fat, viscosity, the number of pathogenic microorganisms, including salmonella, QMAFAnM, coliform bacteria, yeast and moldy fungi, osmophilic yeast.

Results. The introduction of antioxidants has increased the best before date of urbech by 7-14 days. Mild preservatives have had no effect on increasing the best before date of nut butter. The drug Polybiom has increased the best before date of urbech by 21-28 days. The study of the urbech microbiological indicators with obvious signs of spoilage has shown that the number of pathogenic microorganisms, including salmonella, QMAFAnM, coliform bacteria, molds and yeasts does not exceed the values ​​regulated in TR CU 021/2011. Treatment of the urbech with gamma radiation has shown that the radiation dose of more than 2 kGy leads to the change in its organoleptic properties. Osmoresistant microorganisms are present in all the variants. Compared to the control samples, with an increase in the radiation dose, the osmophilic microflora decreases from 10 to 55 times.

Conclusion. During the storage of newly developed types of urbech, it has been found out that its spoilage is not associated with the natural processes of fat oxidation. No microorganisms above the values regulated in TR CU 021 have been found in the urbech. Osmophilic microorganisms develop in the experimental samples of the urbech. Gamma irradiation of urbech at a dosage permissible for food up to 10 kGy reduces the amount of osmophilic microflora up to 55 times. When treated with radiation at a dose of up to 2 kGy, the urbech organoleptic properties are preserved. Accordingly, the treatment with ionizing radiation at the doses up to 2 kGy is effective for preserving organoleptic and microbiological parameters urbechs, including osmophilic yeast, which is not regulated by the regulatory documents in the Russian Federation, for a certain period of storage. To study the causes of the urbech spoilage, further detailed studies of microorganisms and their metabolic products are required.

EDN: XYAEUQ

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Author Biographies

Roza T. Timakova, Ural State Economic University

Doctor of Technical Sciences, Associate Professor

Andrey A. Khlopov, Vyatka State Agrotechnological University

Candidate of Agricultural Sciences

Elena S. Lybenko, Vyatka State Agrotechnological University

Candidate of Agricultural Sciences, Associate Professor

Sergey O. Nikitin, "RCOT "Era" LLC

General Director

References

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Al-Qadiri, H., Amr, A., Al-Holy, M. A., Shahein, M. (2021). Effect of gamma irradiation against microbial spoilage of hummus preserved under refrigerated storage. Food Science and Technology International, 27(7), 598-607. https://doi.org/10.1177/1082013220975891. EDN: https://elibrary.ru/kjvtfd

Esen, E., Turga, Ö. (2023). Prevention of the Growth of Salmonella Spp. and Listeria Spp. in Tahini by Using Antagonistic Microorganisms. Çukurova Tarım Ve Gıda Bilimleri Dergisi, 38(1), 26-39. https://doi.org/10.36846/CJAFS.2023.96. EDN: https://elibrary.ru/hmxuhs

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Loots, M., Chidamba, L., Korsten, L. (2021). Microbial load and prevalence of Escherichia coli and Salmonella spp. in macadamia nut production systems. Journal of Food Protection, 84(6), 1088-1096. https://doi.org/10.4315/JFP-20-238. EDN: https://elibrary.ru/dczecw

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Topcam, H., Coşkun, E., Son, E., Kütük, D., Aytaç, S. A., Mert, B., Ozturk, S., Erdogdu, F. (2023). Microwave decontamination processing of tahini and process design considerations using a computational approach. Innovative Food Science & Emerging Technologies, 86:103137. https://doi.org/10.1016/j.ifset.2023.103377. EDN: https://elibrary.ru/ahugmg

Osaili, T. M., Al-Nabulsi, A. A., Aljaafreh, T. F. (2018). Use of gamma radiation to inactivate stressed Salmonella spp., Escherichia coli O157:H7 and Listeria monocytogenes in tahini halva. Food Microbiology, 278, 20-25. https://doi.org/10.1016/j.ijfoodmicro.2018.04.029

Sánchez-Maldonado, A. F., Lee, A., Farber, J. M. (2018). Methods for the control of foodborne pathogens in low-moisture foods. Annual Review of Food Science and Technology, 9, 177-208. https://doi.org/10.1146/annurev-food-030117-012304. EDN: https://elibrary.ru/vhacny

Olaimat, A. N., Al-Holy, M. A., Abughoush, M. H., Daseh, L., Al-Nabulsi, A. A., Osaili, T. M., Al-Rousan, W., Maghaydah, S., Ayyash, M., Holley, R. A. (2023). Survival of Salmonella enterica and Listeria monocytogenes in date palm paste and syrup at different storage temperatures. Journal of Food Science, 88(7), 2950-2959. https://doi.org/10.1111/1750-3841.16620. EDN: https://elibrary.ru/xbxxgn

Szpinak, V., Ganz, M., Yaron, S. (2022). Factors affecting the thermal resistance of Salmonella Typhimurium in tahin. Food Research International, 155:111088. https://doi.org/10.1016/j.foodres.2022.111088. EDN: https://elibrary.ru/lrpofk

Al-Nabulsi, A. A., Olaimat, A. N., Osaili, T. M., Shaker, R. R., Elabedeen, N. Z., Jaradat, Z. W., Abushelaibi, A., Holley, R. A. (2014). Use of acetic and citric acids to control Salmonella Typhimurium in tahini (sesame paste). Food Microbiology, 42, 102-108. https://doi.org/10.1016/j.fm.2014.02.020

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Timakova, R., Akulich, A., Samuylenko, T. (2021). The role of biotechnology in ensuring the preservation of dry composite mixtures. E3S Web of Conferences, 254:10018. https://doi.org/10.1051/e3sconf/202125410018. EDN: https://elibrary.ru/ugnqm

Timakova, R., Efremova, S., Zuparova, V. (2021). Ways to improve the technological properties of commercial grain and ensure its preservation. AIP Conference Proceedings, 2419:020017. https://doi.org/10.1063/5.0069615. EDN: https://elibrary.ru/hlylxv

Тутельян, В. А. (2012). Химический состав и калорийность российских продуктов питания: Справочник. М.: Дели Принт. 283 с. ISBN: 978-5-905170-20-1. EDN: https://elibrary.ru/qmcskv

Ahmad, F., Mohammad, Z. H., Zaidi, S. F., Ibrahim, S. A. (2023). A comprehensive review on the application of ultrasound for the preservation of fruits and vegetables. Journal of Food Process Engineering, 46(6), 1-27. https://doi.org/10.1111/jfpe.14291. EDN: https://elibrary.ru/uwgvee

Medvid, O. O., Peredera, Zh. O., Shcherbakova, N. S., Peredera, S. B. (2023). Analysis of the Italian honey market. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 25(112), 16-21. https://doi.org/10.32718/nvlvet11202. EDN: https://elibrary.ru/uingcb

Andersen, N. R., Petersen, R. van D, Frost, M. B. (2022). Consumer interest in hummus made from different pulses: Effects of information about origin and variety seeking tendency. International Journal of Gastronomy and Food Science, 29(1):100572. https://doi.org/10.1016/j.ijgfs.2022.100572. EDN: https://elibrary.ru/fxcjkx

Anderson, N. M. (2018). Recent Advances in Low Moisture Food Pasteurization. Current Opinion in Food Science. https://doi.org/10.1016/j.cofs.2018.11.001

Coulombe, G., Tamber, S. (2022). Salmonella enterica Outbreaks Linked to the Consumption of Tahini and Tahini-Based Products. Microorganisms, 10(11):2299. https://doi.org/10.3390/microorganisms10112299. EDN: https://elibrary.ru/jgroig

Mousavi, Z. E., Hunt, K., Koolman, L., Butler, F., Fanning, S. (2023). Cronobacter Species in the Built Food Production Environment: A Review on Persistence, Pathogenicity, Regulation and Detection Methods. Microorganisms, 11, 2-24. https://doi.org/10.3390/microorganisms11061379. EDN: https://elibrary.ru/nsjdza

Davidson, A. (2014). The Oxford Companion to Food. Oxford: University Press. 921 p. (pp. 802-803). https://doi.org/10.1093/acref/9780199677337.001.0001

Lang, E., Rhee, M. S., Gonçalves, M. P. M. B. B., Sant’Ana, A. A. (2023). Desiccation strategies of Cronobacter sakazakii to survive in low moisture foods and environment. Trends in Food Science & Technology. 104241. https://doi.org/10.1016/j.tifs.2023.104241. EDN: https://elibrary.ru/ucorby

Al-Qadiri, H., Amr, A., Al-Holy, M. A., Shahein, M. (2021). Effect of gamma irradiation against microbial spoilage of hummus preserved under refrigerated storage. Food Science and Technology International, 27(7), 598-607. https://doi.org/10.1177/1082013220975891. EDN: https://elibrary.ru/kjvtfd

Esen, E., Turga, Ö. (2023). Prevention of the Growth of Salmonella Spp. and Listeria Spp. in Tahini by Using Antagonistic Microorganisms. Çukurova Tarım Ve Gıda Bilimleri Dergisi, 38(1), 26-39. https://doi.org/10.36846/CJAFS.2023.96. EDN: https://elibrary.ru/hmxuhs

Grasso, E. M., Stam, C. N., Anderson, N. M., Krishnamurthy, K. (2014). Heat and steam treatments. In: The microbiological safety of low water activity foods and spices. Springer. P. 403-426. https://doi.org/10.1007/978-1-4939-2062-4_21

Al-Nabulsi, A. A., Osaili, T. M., Olaimat, A. N., Almasri, W. E., Ayyash, M., Al-Holy, M. A., Jaradat, Z. W., Obaid, R. S., Holley, R. A. (2020). Inactivation of Salmonella spp. in tahini using plant essential oil extracts. Food microbiology, 86:103338. https://doi.org/10.1016/j.fm.2019.103338. EDN: https://elibrary.ru/zrezxq

Olaimat, A. N., Al-Nabulsi, A. A., Osaili, T. M., Al-Holy, M., Ghoush, M. A., Alkhalidy, H., Jaradat, Z. W., Ayyash, M., Holley, R. A. (2022). Inactivation of stressed Salmonella enterica, Escherichia coli O157:H7, and Listeria monocytogenes in hummus using low dose gamma irradiation. Journal of Food Science, 87(2), 845-855. https://doi.org/10.1111/1750-3841.16036. EDN: https://elibrary.ru/pyndjc

Loots, M., Chidamba, L., Korsten, L. (2021). Microbial load and prevalence of Escherichia coli and Salmonella spp. in macadamia nut production systems. Journal of Food Protection, 84(6), 1088-1096. https://doi.org/10.4315/JFP-20-238. EDN: https://elibrary.ru/dczecw

Mainardi, P. H., Bidoia, E. D. (2024). Food safety management: preventive strategies and control of pathogenic microorganisms in food. European Journal of Biological Research, 14(1), 13-32. https://doi.org/10.5281/zenodo.10724672

Topcam, H., Coşkun, E., Son, E., Kütük, D., Aytaç, S. A., Mert, B., Ozturk, S., Erdogdu, F. (2023). Microwave decontamination processing of tahini and process design considerations using a computational approach. Innovative Food Science & Emerging Technologies, 86:103137. https://doi.org/10.1016/j.ifset.2023.103377. EDN: https://elibrary.ru/ahugmg

Osaili, T. M., Al-Nabulsi, A. A., Aljaafreh, T. F. (2018). Use of gamma radiation to inactivate stressed Salmonella spp., Escherichia coli O157:H7 and Listeria monocytogenes in tahini halva. Food Microbiology, 278, 20-25. https://doi.org/10.1016/j.ijfoodmicro.2018.04.029

Sánchez-Maldonado, A. F., Lee, A., Farber, J. M. (2018). Methods for the control of foodborne pathogens in low-moisture foods. Annual Review of Food Science and Technology, 9, 177-208. https://doi.org/10.1146/annurev-food-030117-012304. EDN: https://elibrary.ru/vhacny

Olaimat, A. N., Al-Holy, M. A., Abughoush, M. H., Daseh, L., Al-Nabulsi, A. A., Osaili, T. M., Al-Rousan, W., Maghaydah, S., Ayyash, M., Holley, R. A. (2023). Survival of Salmonella enterica and Listeria monocytogenes in date palm paste and syrup at different storage temperatures. Journal of Food Science, 88(7), 2950-2959. https://doi.org/10.1111/1750-3841.16620. EDN: https://elibrary.ru/xbxxgn

Szpinak, V., Ganz, M., Yaron, S. (2022). Factors affecting the thermal resistance of Salmonella Typhimurium in tahin. Food Research International, 155:111088. https://doi.org/10.1016/j.foodres.2022.111088. EDN: https://elibrary.ru/lrpofk

Al-Nabulsi, A. A., Olaimat, A. N., Osaili, T. M., Shaker, R. R., Elabedeen, N. Z., Jaradat, Z. W., Abushelaibi, A., Holley, R. A. (2014). Use of acetic and citric acids to control Salmonella Typhimurium in tahini (sesame paste). Food Microbiology, 42, 102-108. https://doi.org/10.1016/j.fm.2014.02.020


Published
2025-08-31
How to Cite
Timakova, R., Khlopov, A., Lybenko, E., & Nikitin, S. (2025). Study of the effect of gamma radiation on the antibiotic activity of osmotic microbiota in some types of urbechs. Siberian Journal of Life Sciences and Agriculture, 17(3), 424-452. https://doi.org/10.12731/2658-6649-2025-17-3-1133
Section
Interdisciplinary Research