Study of the effect of gamma radiation on the antibiotic activity of osmotic microbiota in some types of urbechs
Abstract
Background. “Urbech” – traditional national product of Dagestan peoples recently found increasing popularity among adherents of a healthy diet. Urbech made according to traditional recipes retains its properties when stored for more than 1-2 years. Nowadays urbech range is constantly expanding. Some of the new types of urbech can spoil in 5-7 days.
Materials and methods. The samples of urbech made from coconut flakes and dried mulberries spoiled most quickly, peanut urbech with grated cocoa beans and date syrup, and sesame urbech with honey. The urbech has been treated with antioxidants and mild preservatives and has been exposed to gamma radiation. Using standard methods, the authors have determined humidity, pH, acid number of fat, viscosity, the number of pathogenic microorganisms, including salmonella, QMAFAnM, coliform bacteria, yeast and moldy fungi, osmophilic yeast.
Results. The introduction of antioxidants has increased the best before date of urbech by 7-14 days. Mild preservatives have had no effect on increasing the best before date of nut butter. The drug Polybiom has increased the best before date of urbech by 21-28 days. The study of the urbech microbiological indicators with obvious signs of spoilage has shown that the number of pathogenic microorganisms, including salmonella, QMAFAnM, coliform bacteria, molds and yeasts does not exceed the values regulated in TR CU 021/2011. Treatment of the urbech with gamma radiation has shown that the radiation dose of more than 2 kGy leads to the change in its organoleptic properties. Osmoresistant microorganisms are present in all the variants. Compared to the control samples, with an increase in the radiation dose, the osmophilic microflora decreases from 10 to 55 times.
Conclusion. During the storage of newly developed types of urbech, it has been found out that its spoilage is not associated with the natural processes of fat oxidation. No microorganisms above the values regulated in TR CU 021 have been found in the urbech. Osmophilic microorganisms develop in the experimental samples of the urbech. Gamma irradiation of urbech at a dosage permissible for food up to 10 kGy reduces the amount of osmophilic microflora up to 55 times. When treated with radiation at a dose of up to 2 kGy, the urbech organoleptic properties are preserved. Accordingly, the treatment with ionizing radiation at the doses up to 2 kGy is effective for preserving organoleptic and microbiological parameters urbechs, including osmophilic yeast, which is not regulated by the regulatory documents in the Russian Federation, for a certain period of storage. To study the causes of the urbech spoilage, further detailed studies of microorganisms and their metabolic products are required.
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References
Yagafar, M. K., Gainutdinov, T. R., Idrisov, A. M., Rakhmatullina, G. I., Yunusov, I. R. (2022). Assessment of biological safety of irradiated feed and food products. Veterinary Doctor, (2), 21-28. https://doi.org/10.33632/1998-698 EDN: https://elibrary.ru/thkguc
Bulatova, A. G., Sergeeva, G. A. (2021). Traditional food culture of the peoples of Dagestan. Moscow: Prospekt. 96 p. ISBN: 978-5-392-34147-4. EDN: https://elibrary.ru/sjdyb
Danyo, E. K., Ivantsova, M. N., Selezneva, I. S. (2024). Ionizing radiation effects on microorganisms and its applications in the food industry. Foods and Raw Materials, 12(1), 1-12. https://doi.org/10.21603/2308-4057-2024-1-583 EDN: https://elibrary.ru/xicyhk
Kushch, I. V., Vanner, N. E., Udavliev, D. I., Muradova, M. D. (2019). Microbiological safety of honey. Health, Food & Biotechnology, 1(3), 106-117. https://doi.org/10.36107/hfb.2019.i3.s267 EDN: https://elibrary.ru/syvgzy
Mordkovich, Ya. B., Baranova, L. I. (2023). Prospects for using radiation disinsection for quarantine phytosanitary disinfection. Phytosanitary. Plant Quarantine, (3), 60-64. https://doi.org/10.69536/u8906-0079-0762-a EDN: https://elibrary.ru/xrbxzc
Ramazanova, Z. B., Gadzhalova, F. A. (2023). Dagestan traditional dishes and food products as a marker of folk culture and regional brand. Heritage of Ages, (2), 31-45. https://doi.org/10.36343/SB.2023.34.2.002 EDN: https://elibrary.ru/iytxow
Sarukhanov, V. Ya., Shesterikov, A. Yu., Pomyasova, M. G., Kharlamov, V. A., Polyakova, I. V., Karpenko, E. I. (2022). Systematization of experimental research results and creation of the database «Radiation processing of agricultural raw materials and food products». Radiation and Risk (Bulletin of the National Radiation and Epidemiological Register), 31(3), 37-47. https://doi.org/10.21870/0131-3878-2022-31-3-37-47 EDN: https://elibrary.ru/adzlir
Nechaev, A. P., Samoylov, A. V., Bessonov, V. V., Nikolaeva, Yu. V., Tarasova, V. V., Pilipenko, O. V. (2020). Influence of antioxidants in native and micellated forms on shelf life of emulsion fat product. Voprosy Pitania, 89(5), 101-109. https://doi.org/10.24411/0042-8833-2020-10070 EDN: https://elibrary.ru/wwgvza
Bliznyuk, U. A., Borshchegovskaya, P. Yu., Zubritskaya, Ya. V., Ipatova, V. S., Malyuga, A. A., Rozanov, V. V., Chernyaev, A. P., Chulikova, N. S., Yurov, D. S. (2023). Effect of ionizing radiation on germination and biometric indicators of oilseed crops. Technologies of Living Systems, 20(1), 79-89. https://doi.org/10.18127/j.20700997-202301-09 EDN: https://elibrary.ru/ouawqp
Timakova, R. T., Tikhonov, S. L., Tikhonova, N. V. (2020). Ionizing radiation treatment as an innovative process approach in food storage technology for modern agriculture. IOP Conference Series: Earth and Environmental Science, 421(2):022015. https://doi.org/10.1088/1755-1315/421/2/022015. EDN: https://elibrary.ru/uxncvj
Timakova, R., Akulich, A., Samuylenko, T. (2021). The role of biotechnology in ensuring the preservation of dry composite mixtures. E3S Web of Conferences, 254:10018. https://doi.org/10.1051/e3sconf/202125410018. EDN: https://elibrary.ru/ugnqm
Timakova, R., Efremova, S., Zuparova, V. (2021). Ways to improve the technological properties of commercial grain and ensure its preservation. AIP Conference Proceedings, 2419:020017. https://doi.org/10.1063/5.0069615. EDN: https://elibrary.ru/hlylxv
Тутельян, В. А. (2012). Химический состав и калорийность российских продуктов питания: Справочник. М.: Дели Принт. 283 с. ISBN: 978-5-905170-20-1. EDN: https://elibrary.ru/qmcskv
Ahmad, F., Mohammad, Z. H., Zaidi, S. F., Ibrahim, S. A. (2023). A comprehensive review on the application of ultrasound for the preservation of fruits and vegetables. Journal of Food Process Engineering, 46(6), 1-27. https://doi.org/10.1111/jfpe.14291. EDN: https://elibrary.ru/uwgvee
Medvid, O. O., Peredera, Zh. O., Shcherbakova, N. S., Peredera, S. B. (2023). Analysis of the Italian honey market. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 25(112), 16-21. https://doi.org/10.32718/nvlvet11202. EDN: https://elibrary.ru/uingcb
Andersen, N. R., Petersen, R. van D, Frost, M. B. (2022). Consumer interest in hummus made from different pulses: Effects of information about origin and variety seeking tendency. International Journal of Gastronomy and Food Science, 29(1):100572. https://doi.org/10.1016/j.ijgfs.2022.100572. EDN: https://elibrary.ru/fxcjkx
Anderson, N. M. (2018). Recent Advances in Low Moisture Food Pasteurization. Current Opinion in Food Science. https://doi.org/10.1016/j.cofs.2018.11.001
Coulombe, G., Tamber, S. (2022). Salmonella enterica Outbreaks Linked to the Consumption of Tahini and Tahini-Based Products. Microorganisms, 10(11):2299. https://doi.org/10.3390/microorganisms10112299. EDN: https://elibrary.ru/jgroig
Mousavi, Z. E., Hunt, K., Koolman, L., Butler, F., Fanning, S. (2023). Cronobacter Species in the Built Food Production Environment: A Review on Persistence, Pathogenicity, Regulation and Detection Methods. Microorganisms, 11, 2-24. https://doi.org/10.3390/microorganisms11061379. EDN: https://elibrary.ru/nsjdza
Davidson, A. (2014). The Oxford Companion to Food. Oxford: University Press. 921 p. (pp. 802-803). https://doi.org/10.1093/acref/9780199677337.001.0001
Lang, E., Rhee, M. S., Gonçalves, M. P. M. B. B., Sant’Ana, A. A. (2023). Desiccation strategies of Cronobacter sakazakii to survive in low moisture foods and environment. Trends in Food Science & Technology. 104241. https://doi.org/10.1016/j.tifs.2023.104241. EDN: https://elibrary.ru/ucorby
Al-Qadiri, H., Amr, A., Al-Holy, M. A., Shahein, M. (2021). Effect of gamma irradiation against microbial spoilage of hummus preserved under refrigerated storage. Food Science and Technology International, 27(7), 598-607. https://doi.org/10.1177/1082013220975891. EDN: https://elibrary.ru/kjvtfd
Esen, E., Turga, Ö. (2023). Prevention of the Growth of Salmonella Spp. and Listeria Spp. in Tahini by Using Antagonistic Microorganisms. Çukurova Tarım Ve Gıda Bilimleri Dergisi, 38(1), 26-39. https://doi.org/10.36846/CJAFS.2023.96. EDN: https://elibrary.ru/hmxuhs
Grasso, E. M., Stam, C. N., Anderson, N. M., Krishnamurthy, K. (2014). Heat and steam treatments. In: The microbiological safety of low water activity foods and spices. Springer. P. 403-426. https://doi.org/10.1007/978-1-4939-2062-4_21
Al-Nabulsi, A. A., Osaili, T. M., Olaimat, A. N., Almasri, W. E., Ayyash, M., Al-Holy, M. A., Jaradat, Z. W., Obaid, R. S., Holley, R. A. (2020). Inactivation of Salmonella spp. in tahini using plant essential oil extracts. Food microbiology, 86:103338. https://doi.org/10.1016/j.fm.2019.103338. EDN: https://elibrary.ru/zrezxq
Olaimat, A. N., Al-Nabulsi, A. A., Osaili, T. M., Al-Holy, M., Ghoush, M. A., Alkhalidy, H., Jaradat, Z. W., Ayyash, M., Holley, R. A. (2022). Inactivation of stressed Salmonella enterica, Escherichia coli O157:H7, and Listeria monocytogenes in hummus using low dose gamma irradiation. Journal of Food Science, 87(2), 845-855. https://doi.org/10.1111/1750-3841.16036. EDN: https://elibrary.ru/pyndjc
Loots, M., Chidamba, L., Korsten, L. (2021). Microbial load and prevalence of Escherichia coli and Salmonella spp. in macadamia nut production systems. Journal of Food Protection, 84(6), 1088-1096. https://doi.org/10.4315/JFP-20-238. EDN: https://elibrary.ru/dczecw
Mainardi, P. H., Bidoia, E. D. (2024). Food safety management: preventive strategies and control of pathogenic microorganisms in food. European Journal of Biological Research, 14(1), 13-32. https://doi.org/10.5281/zenodo.10724672
Topcam, H., Coşkun, E., Son, E., Kütük, D., Aytaç, S. A., Mert, B., Ozturk, S., Erdogdu, F. (2023). Microwave decontamination processing of tahini and process design considerations using a computational approach. Innovative Food Science & Emerging Technologies, 86:103137. https://doi.org/10.1016/j.ifset.2023.103377. EDN: https://elibrary.ru/ahugmg
Osaili, T. M., Al-Nabulsi, A. A., Aljaafreh, T. F. (2018). Use of gamma radiation to inactivate stressed Salmonella spp., Escherichia coli O157:H7 and Listeria monocytogenes in tahini halva. Food Microbiology, 278, 20-25. https://doi.org/10.1016/j.ijfoodmicro.2018.04.029
Sánchez-Maldonado, A. F., Lee, A., Farber, J. M. (2018). Methods for the control of foodborne pathogens in low-moisture foods. Annual Review of Food Science and Technology, 9, 177-208. https://doi.org/10.1146/annurev-food-030117-012304. EDN: https://elibrary.ru/vhacny
Olaimat, A. N., Al-Holy, M. A., Abughoush, M. H., Daseh, L., Al-Nabulsi, A. A., Osaili, T. M., Al-Rousan, W., Maghaydah, S., Ayyash, M., Holley, R. A. (2023). Survival of Salmonella enterica and Listeria monocytogenes in date palm paste and syrup at different storage temperatures. Journal of Food Science, 88(7), 2950-2959. https://doi.org/10.1111/1750-3841.16620. EDN: https://elibrary.ru/xbxxgn
Szpinak, V., Ganz, M., Yaron, S. (2022). Factors affecting the thermal resistance of Salmonella Typhimurium in tahin. Food Research International, 155:111088. https://doi.org/10.1016/j.foodres.2022.111088. EDN: https://elibrary.ru/lrpofk
Al-Nabulsi, A. A., Olaimat, A. N., Osaili, T. M., Shaker, R. R., Elabedeen, N. Z., Jaradat, Z. W., Abushelaibi, A., Holley, R. A. (2014). Use of acetic and citric acids to control Salmonella Typhimurium in tahini (sesame paste). Food Microbiology, 42, 102-108. https://doi.org/10.1016/j.fm.2014.02.020
Список литературы
Ягафар, М. К., Гайнутдинов, Т. Р., Идрисов, А. М., Рахматуллина, Г. И., Юнусов, И. Р. (2022). Оценка биологической безопасности облученных кормов и пищевых продуктов. Ветеринарный врач, (2), 21-28. https://doi.org/10.33632/1998-698. EDN: https://elibrary.ru/thkguc
Булатова, А. Г., Сергеева, Г. А. (2021). Традиционная культура питания народов Дагестана. М.: Проспект. 96 с. ISBN: 978-5-392-34147-4. EDN: https://elibrary.ru/sjdyb
Danyo, E. K., Ivantsova, M. N., Selezneva, I. S. (2024). Ionizing radiation effects on microorganisms and its applications in the food industry. Foods and Raw Materials, 12(1), 1-12. https://doi.org/10.21603/2308-4057-2024-1-583. EDN: https://elibrary.ru/xicyhk
Kushch, I. V., Vanner, N. E., Udavliev, D. I., Muradova, M. D. (2019). Microbiological Safety of Honey. Health, Food & Biotechnology, 1(3), 106-117. https://doi.org/10.36107/hfb.2019.i3.s267. EDN: https://elibrary.ru/syvgzy
Мордкович, Я. Б., Баранова, Л. И. (2023). Перспективы использования радиационной дезинсекции для карантинного фитосанитарного обеззараживания. Фитосанитария. Карантин растений, (3), 60-64. https://doi.org/10.69536/u8906-0079-0762-a. EDN: https://elibrary.ru/xrbxzc
Рамазанова, З. Б., Гаджалова, Ф. А. (2023). Дагестанские традиционные блюда и продукты питания как маркер народной культуры и бренд региона. Наследие веков, (2), 31-45. https://doi.org/10.36343/SB.2023.34.2.002. EDN: https://elibrary.ru/iytxow
Саруханов, В. Я., Шестериков, А. Ю., Помясова, М. Г., Харламов, В. А., Полякова, И. В., Карпенко, Е. И. (2022). Систематизация результатов экспериментальных исследований и создание базы данных «Радиационная обработка сельскохозяйственного сырья и пищевой продукции». Радиация и риск (Бюллетень Национального радиационно-эпидемиологического регистра), 31(3), 37-47. https://doi.org/10.21870/0131-3878-2022-31-3-37-47. EDN: https://elibrary.ru/adzlir
Нечаев, А. П., Самойлов, А. В., Бессонов, В. В., Николаева, Ю. В., Тарасова, В. В., Пилипенко, О. В. (2020). Влияние антиоксидантов в нативной и мицеллированной формах на сроки годности эмульсионного жирового продукта. Вопросы питания, 89(5), 101-109. https://doi.org/10.24411/0042-8833-2020-10070. EDN: https://elibrary.ru/wwgvza
Близнюк, У. А., Борщеговская, П. Ю., Зубрицкая, Я. В., Ипатова, В. С., Малюга, А. А., Розанов, В. В., Черняев, А. П., Чуликова, Н. С., Юров, Д. С. (2023). Влияние ионизирующего излучения на всхожесть и биометрические показатели масличных культур. Технологии живых систем, 20(1), 79-89. https://doi.org/10.18127/j.20700997-202301-09. EDN: https://elibrary.ru/ouawqp
Timakova, R. T., Tikhonov, S. L., Tikhonova, N. V. (2020). Ionizing radiation treatment as an innovative process approach in food storage technology for modern agriculture. IOP Conference Series: Earth and Environmental Science, 421(2):022015. https://doi.org/10.1088/1755-1315/421/2/022015. EDN: https://elibrary.ru/uxncvj
Timakova, R., Akulich, A., Samuylenko, T. (2021). The role of biotechnology in ensuring the preservation of dry composite mixtures. E3S Web of Conferences, 254:10018. https://doi.org/10.1051/e3sconf/202125410018. EDN: https://elibrary.ru/ugnqm
Timakova, R., Efremova, S., Zuparova, V. (2021). Ways to improve the technological properties of commercial grain and ensure its preservation. AIP Conference Proceedings, 2419:020017. https://doi.org/10.1063/5.0069615. EDN: https://elibrary.ru/hlylxv
Тутельян, В. А. (2012). Химический состав и калорийность российских продуктов питания: Справочник. М.: Дели Принт. 283 с. ISBN: 978-5-905170-20-1. EDN: https://elibrary.ru/qmcskv
Ahmad, F., Mohammad, Z. H., Zaidi, S. F., Ibrahim, S. A. (2023). A comprehensive review on the application of ultrasound for the preservation of fruits and vegetables. Journal of Food Process Engineering, 46(6), 1-27. https://doi.org/10.1111/jfpe.14291. EDN: https://elibrary.ru/uwgvee
Medvid, O. O., Peredera, Zh. O., Shcherbakova, N. S., Peredera, S. B. (2023). Analysis of the Italian honey market. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 25(112), 16-21. https://doi.org/10.32718/nvlvet11202. EDN: https://elibrary.ru/uingcb
Andersen, N. R., Petersen, R. van D, Frost, M. B. (2022). Consumer interest in hummus made from different pulses: Effects of information about origin and variety seeking tendency. International Journal of Gastronomy and Food Science, 29(1):100572. https://doi.org/10.1016/j.ijgfs.2022.100572. EDN: https://elibrary.ru/fxcjkx
Anderson, N. M. (2018). Recent Advances in Low Moisture Food Pasteurization. Current Opinion in Food Science. https://doi.org/10.1016/j.cofs.2018.11.001
Coulombe, G., Tamber, S. (2022). Salmonella enterica Outbreaks Linked to the Consumption of Tahini and Tahini-Based Products. Microorganisms, 10(11):2299. https://doi.org/10.3390/microorganisms10112299. EDN: https://elibrary.ru/jgroig
Mousavi, Z. E., Hunt, K., Koolman, L., Butler, F., Fanning, S. (2023). Cronobacter Species in the Built Food Production Environment: A Review on Persistence, Pathogenicity, Regulation and Detection Methods. Microorganisms, 11, 2-24. https://doi.org/10.3390/microorganisms11061379. EDN: https://elibrary.ru/nsjdza
Davidson, A. (2014). The Oxford Companion to Food. Oxford: University Press. 921 p. (pp. 802-803). https://doi.org/10.1093/acref/9780199677337.001.0001
Lang, E., Rhee, M. S., Gonçalves, M. P. M. B. B., Sant’Ana, A. A. (2023). Desiccation strategies of Cronobacter sakazakii to survive in low moisture foods and environment. Trends in Food Science & Technology. 104241. https://doi.org/10.1016/j.tifs.2023.104241. EDN: https://elibrary.ru/ucorby
Al-Qadiri, H., Amr, A., Al-Holy, M. A., Shahein, M. (2021). Effect of gamma irradiation against microbial spoilage of hummus preserved under refrigerated storage. Food Science and Technology International, 27(7), 598-607. https://doi.org/10.1177/1082013220975891. EDN: https://elibrary.ru/kjvtfd
Esen, E., Turga, Ö. (2023). Prevention of the Growth of Salmonella Spp. and Listeria Spp. in Tahini by Using Antagonistic Microorganisms. Çukurova Tarım Ve Gıda Bilimleri Dergisi, 38(1), 26-39. https://doi.org/10.36846/CJAFS.2023.96. EDN: https://elibrary.ru/hmxuhs
Grasso, E. M., Stam, C. N., Anderson, N. M., Krishnamurthy, K. (2014). Heat and steam treatments. In: The microbiological safety of low water activity foods and spices. Springer. P. 403-426. https://doi.org/10.1007/978-1-4939-2062-4_21
Al-Nabulsi, A. A., Osaili, T. M., Olaimat, A. N., Almasri, W. E., Ayyash, M., Al-Holy, M. A., Jaradat, Z. W., Obaid, R. S., Holley, R. A. (2020). Inactivation of Salmonella spp. in tahini using plant essential oil extracts. Food microbiology, 86:103338. https://doi.org/10.1016/j.fm.2019.103338. EDN: https://elibrary.ru/zrezxq
Olaimat, A. N., Al-Nabulsi, A. A., Osaili, T. M., Al-Holy, M., Ghoush, M. A., Alkhalidy, H., Jaradat, Z. W., Ayyash, M., Holley, R. A. (2022). Inactivation of stressed Salmonella enterica, Escherichia coli O157:H7, and Listeria monocytogenes in hummus using low dose gamma irradiation. Journal of Food Science, 87(2), 845-855. https://doi.org/10.1111/1750-3841.16036. EDN: https://elibrary.ru/pyndjc
Loots, M., Chidamba, L., Korsten, L. (2021). Microbial load and prevalence of Escherichia coli and Salmonella spp. in macadamia nut production systems. Journal of Food Protection, 84(6), 1088-1096. https://doi.org/10.4315/JFP-20-238. EDN: https://elibrary.ru/dczecw
Mainardi, P. H., Bidoia, E. D. (2024). Food safety management: preventive strategies and control of pathogenic microorganisms in food. European Journal of Biological Research, 14(1), 13-32. https://doi.org/10.5281/zenodo.10724672
Topcam, H., Coşkun, E., Son, E., Kütük, D., Aytaç, S. A., Mert, B., Ozturk, S., Erdogdu, F. (2023). Microwave decontamination processing of tahini and process design considerations using a computational approach. Innovative Food Science & Emerging Technologies, 86:103137. https://doi.org/10.1016/j.ifset.2023.103377. EDN: https://elibrary.ru/ahugmg
Osaili, T. M., Al-Nabulsi, A. A., Aljaafreh, T. F. (2018). Use of gamma radiation to inactivate stressed Salmonella spp., Escherichia coli O157:H7 and Listeria monocytogenes in tahini halva. Food Microbiology, 278, 20-25. https://doi.org/10.1016/j.ijfoodmicro.2018.04.029
Sánchez-Maldonado, A. F., Lee, A., Farber, J. M. (2018). Methods for the control of foodborne pathogens in low-moisture foods. Annual Review of Food Science and Technology, 9, 177-208. https://doi.org/10.1146/annurev-food-030117-012304. EDN: https://elibrary.ru/vhacny
Olaimat, A. N., Al-Holy, M. A., Abughoush, M. H., Daseh, L., Al-Nabulsi, A. A., Osaili, T. M., Al-Rousan, W., Maghaydah, S., Ayyash, M., Holley, R. A. (2023). Survival of Salmonella enterica and Listeria monocytogenes in date palm paste and syrup at different storage temperatures. Journal of Food Science, 88(7), 2950-2959. https://doi.org/10.1111/1750-3841.16620. EDN: https://elibrary.ru/xbxxgn
Szpinak, V., Ganz, M., Yaron, S. (2022). Factors affecting the thermal resistance of Salmonella Typhimurium in tahin. Food Research International, 155:111088. https://doi.org/10.1016/j.foodres.2022.111088. EDN: https://elibrary.ru/lrpofk
Al-Nabulsi, A. A., Olaimat, A. N., Osaili, T. M., Shaker, R. R., Elabedeen, N. Z., Jaradat, Z. W., Abushelaibi, A., Holley, R. A. (2014). Use of acetic and citric acids to control Salmonella Typhimurium in tahini (sesame paste). Food Microbiology, 42, 102-108. https://doi.org/10.1016/j.fm.2014.02.020
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