CHANGES IN THE METABOLISM OF NATIVE AND DECONSERVED BULL SPERMATOZOA UNDER THE ACTION OF MOLECULAR HYDROGEN
Abstract
Background. The process of storing sperm in a deeply frozen state causes structural and functional disorders of spermatozoa, which reduces their fertilizing ability. The main mechanism of the negative effect of cryopreservation on spermatozoa is the development of oxidative stress.
Molecular hydrogen has some advantages as a potential antioxidant molecule - it is a selective effect on certain reactive oxygen species, the ability to overcome cell membranes, the absence of toxic effects.
Purpose. To study the effect of molecular hydrogen on the functional status of native and deconserved sperm cells of breeding bulls.
Materials and methods. The object of the study was the ejaculates of bulls before cryopreservation and after defrosting. The native sperm diluted with the "BioXcell" diluent, the native sperm diluted with the "BioXcell" diluent enriched with molecular hydrogen, the sperm after deep freezing and the sperm after deep freezing pretreated with molecular hydrogen were studied. The intensity of free radical processes and the activity of antioxidant enzymes were determined in spermatozoa.
Results. After cryopreservation of sperm, the processes of lipid peroxidation in spermatozoa are significantly activated. The content of malondialdehyde, diene and triene conjugates increases. The use of molecular hydrogen to correct the quality of sperm production after cryopreservation gave positive results. A decrease in the concentration of primary and intermediate products of lipid peroxidation was noted. The activity of superoxide dismutase and catalase in spermatozoa significantly increases.
Conclusion. Molecular hydrogen has the potential as a new and effective antioxidant, the widespread use of which is possible for veterinary purposes.
Sponsorship information. The study was funded by the Russian Science Foundation grant No. 23-26-00205.
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Nongbua T. Adding bovine seminal plasma prior to freezing improves post-thaw bull sperm kinematics but decreases mitochondrial activity. Systems biology in reproductive medicine, 2018, vol. 64, no. 3, pp. 183-190.
Ohta S. Recent progress toward hydrogen medicine: potential of molecular hydrogen for preventive and therapeutic application. Current Pharmaceutical Design, 2017, vol. 17 (22), pp. 2241-2252.
Ohta S. Molecular hydrogen as a preventive and therapeutic medical gas: initiation, development and potential of hydrogen medicine. Pharmacology & Therapeutics, 2014, vol. 144 (1), pp. 1–11.
Ohta S. Molecular hydrogen as a novel antioxidant: Overview of the advantages of hydrogen for medical applications. Methods in Enzymology, 2015, vol. 555, pp. 289-317.
Ollero M. et al. Variation in docosahexaenoic acid content in subsets of human spermatozoa at different stages of maturation: implications for sperm lipoperoxidative damage / M. Ollero, R.D. Powers, J.G. Alvarez. Mol. Reprod. Devel., 2000, vol. 55, pp. 326-334.
Pahune P.P. et al. The total antioxidant power of semen and its correlation with the fertility potential of human male subjects / P.P. Pahune, A.R. Choudhari, P.A. Muley. J. Clin. Diagn. Res., 2013, vol. 7(6), pp. 991-995.
Paudel B. et al. Sperm capacitation is associated with phosphorylation of the testis-specific radial spoke protein Rspha / B. Paudel, M.G. Gervasi, J. Porambo, D. Caraballo. Biology of Reproduction, 2018, vol. 100, no. 2, pp. 440-454.
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