ACTIVITY OF OXYGEN RADICALS IN HORSES UNDER PHYSICAL LOADS OF DIFFERENT INTENSITIES
Abstract
The aim of the study was to study the activity of prooxidants, oxidants and antioxidants in sports horses before and after intense physical activity of varying intensity. Neutrophilic granulocytes, blood serum and saliva were collected from 21 dressage Trakehner sports horses aged 4 to 18 years. Horses were tested before and after training at different intensities (low, medium and high). Respiratory rate, heart rate, electrocardiogram, hematological and biochemical blood parameters were determined. Primary, secondary and tertiary oxygen radicals in the samples were assessed using chemiluminescence analysis. As a result of the study, an increased activity of the oxidative and antioxidant enzymatic systems was revealed at minimum and medium loads and of the antioxidant system at maximum physical exertion. Horses adapt to the increased physical activity. As a result, there is no change in the activity of free radicals (prooxidants and oxidants). At the same time, the systolic indicator of horses, the P wave, was significantly increased with low physical activity relative to the indicators of average intensity. Hematological and biochemical blood parameters before and after training were within the physiological norm. The amount of hemoglobin was higher at low exercise and lower at high exercise. The number of erythrocytes decreased with increasing intensity of physical activity, and the number of leukocytes increased. An increase in total protein and a decrease in glucose concentration with an increase in the intensity of physical activity were revealed.
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