EFFECT OF LIGHT DESYNCHRONIZATION ON STRESS-INDUCING AND STRESS-BLOCKING PHYSIOLOGICAL MECHANISMS IN AGED MICE
Abstract
Purpose. Investigation of the mechanisms of formation of changes in glucocorticoid reception (in an experiment on white outbred mice of old age) under the influence of light desynchronization.
Materials and methods. The objects of the study were white outbred mice (n = 246), the average age of which was about 19 months. Light desynchronization was simulated by changing the lighting mode (a combination of natural and artificial lighting) for 23 days. On certain days after the onset of desynchronization, blood samples were taken, in which the plasma cortisol level was determined using the Multiskan GO enzyme immunoassay analyzer. The radioimmunological method was used to detect antigen-antibody complexes in which one of the immunoreagents was labeled with a radioactive isotope. The results were analyzed to identify similarities or differences (a two-sample Student’s t test was used, with an assessment of the significance of differences at p <0.05).
Results and discussion. The results of analysis of the expression level of type II glucocorticoid receptors (referred to as stress-inducing factors) and the expression level of type III glucocorticoid receptors (stress-blocking factor) at various times after light desynchronization indicated a pathological decrease in adaptive abilities.In old mice, this was expressed in an increase in the number of stress-inducing factors and an inadequate decrease in the synthesis of stress-blocking factors. Probably, in old mice, in response to stressful stimuli, the protective mechanisms of their organism are weakened. The data obtained do not contradict the generally accepted concepts that interpret the role of the glucocorticoid system in the system of protective mechanisms of the body. However, it is known that in addition to glucocorticoid and antiglucocorticoid mechanisms, there are other links that react during light desynchronization (the level of cytokines, endogenous opioids, serotonin, etc.). In this context, we planned to continue studies on even more animals and, along with the study of glucocorticoid reception during light desynchronization, also to carry out a comprehensive analysis of indicators of the immune and neurotransmitter status.
Conclusion. Studies of the effect of light desynchronization on stress-inducing and stress-blocking physiological mechanisms in old mice revealed a high degree of stress response in them to this stimulus. This information can be very useful in medicine and biology. At the same time, for more reliable conclusions, it is necessary to continue studies on even more animals (including young ones) and to study, along with glucocorticoids, indicators of the immune and neurotransmitter status.
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