STUDY OF THE DISTRIBUTION OF LARVAE IN MUSCLES AND SOME PERIPHERAL BLOOD PARAMETERS OF GALLUS GALLUS IN EXPERIMENTAL INFECTION WITH TRICHINELLA PSEUDOSPIRALIS
Abstract
Background. Poultry meat, the main host of the capsule-free species, is the most popular among the population. The consumer willingly buys both meat of chickens, laying hens, ducks, turkeys and geese, and hog game. However, to date there are no specific indications for the procedure for diagnosing poultry meat for trichinosis. In this regard, the scientific novelty of this study was the clarification of the groups of affected muscles during the experimental invasion of Gallus gallus, since the main danger to humans is the absence of characteristic clinical signs of trichinosis in birds.
The aim of the study was to study the distribution of larvae in the muscles and some indicators of peripheral blood of birds during experimental infection with Trichinella pseudospiralis.
Materials and methods. The birds of the experimental group were weighed and T. pseudospiralis larvae isolate was injected into the tooth at a dose of 2 lich./ g of body weight (3600±400 larvae per bird). T. pseudospiralis larvae used for experimental infection of poultry were originally isolated from the muscle tissue of poultry, and were maintained on laboratory animals. In order to determine the distribution of larvae in the muscles of T. pseudospiralis poultry, 6 chickens of the experimental group were euthanized 3,5-4 months after experimental infection. The birds were removed from the experiment in accordance with the "Rules for carrying out work using experimental animals" and in accordance with the principles of the Helsinki Declaration of the World Medical Association (Declaration of Helsinki, and approved by the Institutional Review Board). The objects of the study were microscopized at magnification x 20 and x 80, with the production of photographs (Vision Bio, 2014). The data obtained were processed using standard MS Excel and Statgraphics software packages.
Research results. The average of the intensity of the invasion was 1570±340 l/g of muscles from muscle samples of the studied groups. The indicators of red blood for 30 weeks changed slightly were at the lower limit of the physiological norm. During the study of compression trichinelloscopy of 72 sections, it was found that the largest numbers of larvae were found in the muscles of the head and tongue, less in the muscles of the neck and lower leg, no larvae were found in the chest muscles during this infection.
Conclusion. In conclusion, it should be noted that currently trichinelloscopic control should be standardized in the agro-industrial complex. It is necessary to examine the muscles of the head and neck of birds, which in our studies were most heavily invaded by trichinella, and pay special attention to the edges of the sample sections.
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References
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Zhdanova O., Haidarova A.A., Napisanova L.A., Rossohin D., Lozhenicina O.The possibility of using trichinella spiralis as an experimental model in the field of high dilutions. International Journal of High Dilution Research, 2015, vol. 14, no. 2, pp. 60-61.
Zhdanova O.B., Rudneva O.V., Akulinina Yu.K., Napisanova L.A. Еvaluation of the effectiveness of different immunostimulatory medicine at the experimental trichinosis and leishmaniosis on white mice. International Journal of High Dilution Research, 2019, vol. 18, no. 2, pp. 12.
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