The effect of oxidative stress on epigenetic regulation of gene expression in intestinal cancer
Аннотация
Background. Cancer is caused by the accumulation of genetic and epigenetic changes in the oncogenes and tumor-substituting genes. The discovery of the epigenetic pathways that control gene expression has substantially progressed in understanding cancer biology.
Purpose. Investigation of epigenetic effects of oxidative stress on the regulation of gene expression in intestinal cancer.
Methods. DLD-1 colorectal adenocarcinoma cells were treated with the oxidant 2,2'-Azobis (2-amidinopropane) dihydrochloride (AAPH) and the antioxidant hydroxytyrosol. Cellular viability, apoptosis, reactive oxygen species (ROS) production, and expression of key genes (CDH17, CXCL12, TGFB1) were examined.
Results. Hydroxytyrosol treatment improved DLD-1 cell viability in a time-dependent manner. AAPH-induced oxidative stress decreased cell viability and increased apoptosis and ROS production. Combination treatment with hydroxytyrosol and antioxidant enzymes enhanced these effects. Oxidative stress altered the expression of CDH17, CXCL12 and TGFB1, with TGFB1 showing a dose-dependent increase.
Conclusion. Oxidative stress influences epigenetic regulation of gene expression in intestinal cancer cells. Combination therapies targeting both oxidative stress pathways and epigenetic mechanisms may be a promising approach for colorectal cancer treatment.
EDN: IRRXEV
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