XANTHINE OXIDOREDUCTASE: ACTIVITY OF BLOOD IN RHEUMATOID ARTHRITIS
Abstract
Metabolic shifts are essential for pathogenesis of rheumatoid arthritis (RA) along with changes in immunity.
Objective. Overviewing of xanthine oxidoreductase (XOR) activity pattern in plasma and lyzed red blood cells of RA patients.
Methods. 77 RA patients as well as 35 normal control persons were included in the study. Ethical principles of the World Medical Association Declaration of Helsinki were fully compliant during the research. We verified RA diagnosis using ACR/EULAR criteria (2010). Erythrocytes were separated from whole blood by means of density gradients and lyzed by triple freezing and thawing cycles followed by centrifugation. Xanthine oxidase (XO) and xanthine dehydrogenase (XDH) activities of XOR were quantitated by spectrophotometry. Statistic calculations were performed with STATISTICA 6 software.
Results. Both plasma and lyzed red blood cells had changes in XO as well as XDH activities related to clinical manifestations of RA. Gradual decrease of XDH activity along with disease flares were revealed in both investigated compartments. Extraarticular involvement was associated with increase of both enzymatic activities in plasma, increase of erythrocyte XO, and decrease of erythrocyte XDH. Highest oxidase activities were observed in X-ray stages II and III. High global functional classes of RA were characterized by high XO activities in plasma and RBCs as well as low XDH activities in RBCs.
Conclusion. Increased production of reactive oxygen and nitrogen species is considered to be a major pathogenetic implication of XOR activity. Enhancement of free radical oxidation supports chronic inflammation and destruction in joints, as well as in other organs and systems.
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Copyright (c) 2021 Elena E. Mozgovaya, Svetlana A. Bedina, Maria A. Mamus, Andrew S. Trofimenko, Svetlana S. Spitsina
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