Assessment of cytogenetic index and marker of oxidative stress in a monkey goby (Neogobius fluviatilis) native to the Northern Caspian Sea
Abstract
Background. Currently, environmental changes during oil and gas production in the Northern Caspian Sea are controlled through local environmental monitoring, the obligation of which is determined by Federal Standards and Regulations. Biochemical and cytogenetic markers are distinguished among the markers for assessing the state of aquatic organisms.
Purpose. Assessment of cytogenetic parameters and markers of oxidative stress in a monkey goby captured at the sites of liquidated wells by «LUKOIL-Nizhnevolzhskneft».
Materials and methods. The study was conducted on 308 fish specimens. Cytogenetic disorders were investigated by a micronucleus test. The intensity of POL in liver tissues was assessed by the level of accumulation of MDA. The results were processed statistically using Microsoft Office Excel 2007 software.
Results. The average frequency of occurrence of erythrocytes with micronuclei in fish in some areas exceeded the level of spontaneous mutagenesis, and amounted to 0.25%. An increased content of MDA in hepatocytes was noted. The analysis between the indicators of oxidative stress and the number of micronuclei allows us to conclude that the formation of micronuclei after exposure to free radicals may be an adaptive response to this stress factor in living conditions.
Conclusion. The obtained results of biochemical and cytogenetic studies indicate the presence of two simultaneous pathological processes in fish. On the one hand, an increase in the intensity of free radical oxidation processes, on the other, genome instability with an increased number of cytogenetic rearrangements. Consequently, the destabilization of the genome occurs against the background of activation of free radical processes, and with an increase in the level of MDA above 65 nmol, the mutagenic effect of free radicals on the genome increases significantly.
EDN: NPDHVL
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References
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