Seed lipoxygenase activity and agronomic parameters in the substitution wheat lines Chinese Spring (Synthetic 6X) under optimal and deficient water supply
Abstract
Background. The use of introgression of wild cereal genome segments to maintain the yield and functional quality of wheat under water deficit is a topical task in breeding. Of great interest is the introgression of genes of lipoxygenase (LOX), which is involved in determining gluten quality and drought resistance in wheat.
Purpose. To study the effect of introgression of wild cereal chromosomes on the seed LOX activity and its association with agronomic parameters of wheat under different water supply.
Materials and methods. The objects of the study were the wheat lines cv. Chinese Spring (CS) with the substitution of homologous chromosomes from the hexaploid Synthetic 6x (Syn6x), which contains sub genomes from Triticum dicoccoides (AABB) and Aegilops tauschii (DD). The LOX activity and 9 agronomic parameters were studied under optimal water regime and simulated soil drought.
Results. Under drought, LOX activity was positively correlated with the rate of gluten proteins aggregation, grain weight per ear and grain size. Substitution of 4D and 5D chromosomes, carrying known genes of seed LOX contributed to an increase in the aggregation rate and maintenance of a high level of gluten content. At the same time, the productivity of the ear decreased under control, but increased under drought. This effect was most pronounced in the line CS(Sin6x 5D) with high LOX activity. The line Ch(Sin6x 4D) with low LOX activity reduced ear productivity to a lesser extent under optimal water supply, and showed resistance of ear productivity parameters under drought.
Conclusion. The introgression of the 4D chromosome from Ae. tauschii may be useful for the development of wheat genotypes combining high gluten quality and drought resistance without large detrimental effects on grain yield.
EDN: DUQQLH
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References
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Nicholson, P., Rezannor, H. N., & Worland, A. J. (1993). Chromosomal location of resistance to Septoria nodorum in a synthetic hexaploid wheat determined by the study of chromosomal substitution lines in ‘Chinese Spring’ wheat. Plant Breeding, 110(3), 177–184. https://doi.org/10.1111/j.1439-0523.1993.tb00575.x
McFadden, E. S., & Sears, E. R. (1946). The origin of Triticum spelta and its free threshing hexaploid relatives. Journal of Heredity, 37(3), 81–89. https://doi.org/10.1093/oxfordjournals.jhered.a105590. EDN: https://elibrary.ru/IRNIRV
Osipova, S. V., Permyakova, M. D., & Permyakov, A. V. (2012). The role of non prolamine proteins and LMW redox agents in protein folding and polymerization in wheat grains and the influence on baking quality parameters. Journal of Agricultural and Food Chemistry, 60(49), 12 065–12 073. https://doi.org/10.1021/jf303513m. EDN: https://elibrary.ru/RGIJXL
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Viswanath, K. K., Varakumar, P., Pamuru, R. R., Basha, S. J., Mehta, S., & Rao, A. D. (2020). Plant lipoxygenases and their role in plant physiology. Journal of Plant Biology, 63, 83–95. https://doi.org/10.1007/s12374-020-09241-x. EDN: https://elibrary.ru/EQGTDB
Wasternack, C., & Feussner, I. (2018). The oxylipin pathways: Biochemistry and function. Annual Review of Plant Biology, 69, 363–386. https://doi.org/10.1146/annurev-arplant-042817-040440. EDN: https://elibrary.ru/XVGTHV
Zimmerman, D. C., & Vick, B. A. (1970). Hydroperoxide isomerase: A new enzyme of lipid metabolism. Plant Physiology, 46(3), 445–453. https://doi.org/10.1104/pp.46.3.445
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