Preservation of blood form elements under the protection of combined cryopreservative during low-temperature freezing
Abstract
Background. Cryopreservation has found wide application for various purposes but the disadvantages of existing cryoprotective agents lead to the loss of cell functionality, which can negatively affect the further use of preserved material. The development of effective cryopreservatives is an urgent task requiring the search for low-toxic biocompatible cryoagents.
Materials and methods. The study was carried out on 30 donor volunteers aged 18-23 years who were divided into 2 groups. The object of the study is venous blood. The combined cryopreservative consisted of: glycerin, dimethyl sulfoxide, lactulose, sodium adenosine triphosphate, isotonic sodium chloride solution. A general blood test and a computer cytomorphometric study was performed. Statistical processing of the data was carried out using the application program package IBM SPSS Statistic 23.0.
Purpose. The aim is to evaluate the preservation of blood cells form elements under the protection of the developed combined cryopreservative at low-temperature freezing (–80 °C).
Results. In the course of the study under the conditions of blood samples preservation with the use of combined cryopreservative after daily freezing at –80°C the percentage of preservation of blood formations of leukocytes, erythrocytes, platelets was 52,5±2,51%, 78,07±2,24%, 52,9±2,13% respectively in comparison with control samples (p<0,05).
Conclusion. Morphologic and functional preservation of blood formed elements using combined cryopreservative at low-temperature freezing (–80°C) for one day was revealed. Further research in this field will contribute to the efficiency and safety of whole blood cryopreservation, opening new opportunities for medicine and biology.
EDN: CUBZQM
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References
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Yao, J., Shen, L., Chen, Z., Zhang, B., & Zhao, G. (2022). Hydrogel microencapsulation enhances cryopreservation of red blood cells with trehalose. ACS Biomaterials Science & Engineering, 8(5), 2066–2075. https://doi.org/10.1021/acsbiomaterials.2c00051. EDN: https://elibrary.ru/ENJISC
Zhong, Y., McGrath, J. K., & Gong, B. (2021). Dipropinonates of sugar alcohols as water soluble, nontoxic CPAs for DMSO free cell cryopreservation. ACS Biomaterials Science & Engineering, 7(10), 4757–4762. https://doi.org/10.1021/acsbiomaterials.1c00995. EDN: https://elibrary.ru/DKIIJK
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