The use of grain brewer pellet for the cultivation of edible mushroom mycelium

  • Anastasia V. Tretyakova Tula State Lev Tolstoy Pedagogical University
  • Georgiy V. Pestsov Tula State Lev Tolstoy Pedagogical University
  • Olga V. Prokudina Tula State Lev Tolstoy Pedagogical University
  • Anastasia S. Myagkova Tula State Lev Tolstoy Pedagogical University
Keywords: edible fungus, Pleurotus ostreatus, oyster mushroom, Flammulina velutipes, enoki mushrooms, grain brewer pellet, mycelium

Abstract

Background. Currently, there is a shortage of high-quality mycelium of national production in the Russian Federation. This is due to the deficiencies in mycelium cultivation technology and the lack of high-quality and cheap raw materials for the production of substrates. In the existing mycelium cultivation technologies, it is proposed to use grains of various cereal cultivars, which reduces production profitability due to the cost of raw materials and additional grain processing. To solve this problem, it is proposed to use grain brewer pellet, which is the main solid waste of the brewing industry and contains all the necessary substances for growing the mycelium of edible mushrooms.

Purpose. To investigate the possibility of using grain beer pellets in the technology of cultivation of mycelium of edible mushrooms of Plerotus ostreatus and Flammulina velutipes species.

Methods. Solid-substrate cultivation methods were used to study the effect of brewer grain pellet on the growth rate of edible mushroom mycelium. The object of the study were mushrooms Pleurotus ostreatus strain 813 and Flammulina velutipes strain 631 from the Russian National Collection of Industrial Microorganisms of the NRC “Kurchatov Institute” – GosNIIgenetika.

Results. With regard to findings of the conducted scientific research, it was established that it is better to use nutrient media with the addition of grain brewer pellet in concentrations of 10-15% for the mushroom P. ostreatus and 20-25% for the species F. velutipes to grow the mycelium of edible mushrooms. It is more advantageous to cultivate the mycelium of the mushrooms under study in low-density polyethylene bags.

Conclusion. The use of grain brewer pellet for the cultivation of mycelium of edible mushrooma of the species P. ostreatus and F. velutipes makes it possible to grow high-quality mycelium of national production with low cost-price.

EDN: ALNKII

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Author Biographies

Anastasia V. Tretyakova, Tula State Lev Tolstoy Pedagogical University

Research Assistant Center of Technological Excellence "Advanced Chemical and Biotechnology"

Georgiy V. Pestsov, Tula State Lev Tolstoy Pedagogical University

Doctor of Agricultural Sciences, Professor, Head of the Microbiological Laboratory of the Center for Technological Excellence "Advanced Chemical and Biotechnology"

Olga V. Prokudina, Tula State Lev Tolstoy Pedagogical University

Research Assistant Center of Technological Excellence "Advanced Chemical and Biotechnology"

Anastasia S. Myagkova, Tula State Lev Tolstoy Pedagogical University

Research Assistant Center of Technological Excellence "Advanced Chemical and Biotechnology"

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Mussatto, S., Dragone, G., & Roberto, I. (2006). Brewers’ spent grain: Generation, characteristics and potential applications. Journal of Cereal Science, (43), 1–14. https://doi.org/10.1016/j.jcs.2005.06.001

Gregori, A., Svagelj, M., Pahor, B., & Berovic, M. (2008). The use of spent brewery grains for Pleurotus ostreatus cultivation and enzyme production. New Biotechnology, (25), 157–161. https://doi.org/10.1016/j.nbt.2008.08.003. EDN: https://elibrary.ru/YASCGT

Townsley, P. M. (1979). Preparation of commercial products from brewer’s spent grain and trub. MBAA Technical Quarterly, 16(4), 130–134.


Published
2025-12-30
How to Cite
Tretyakova, A., Pestsov, G., Prokudina, O., & Myagkova, A. (2025). The use of grain brewer pellet for the cultivation of edible mushroom mycelium. Siberian Journal of Life Sciences and Agriculture, 17(6-1). https://doi.org/10.12731/2658-6649-2025-17-6-1-1356
Section
Interdisciplinary Research