Effect of extracts of inflorescence Tagetes patula L. on stress resistance of Paramecium caudatum infusoria

Abstract

Background. Today, the content of native biologically active nutrients in the human diet is decreasing due to the specifics of modern technologies for processing agricultural raw materials and food production, as a result, there is a sharply increasing interest in expanding the range of alternative biologically active drugs based on non-traditional domestic herbal raw materials, which dictates the need to involve new analytical control methods to obtain more reliable information about their antioxidant status. To improve the validity of experimental data, it is necessary to implement an integrated approach that involves the use of analytical control methods that complement each other's information. In this case, the most promising is the use of a biological method based on a test reaction of a biomodel, which allows you to get a more reliable assessment of the integral antioxidant activity of the object under study, due to the high sensitivity of the biotest.

Purpose is to study of integral antioxidant activity of preparations of inflorescence preparations of Tagetes patula L. by biotesting method using as a biotest the equispecific infusoria Paramecium caudatum.

Materials and methods. The objects of the study are aqueous extracts of inflorescences of the inflorescences of the prostrate variety ‘Carmen’, obtained under the following extraction conditions: extractant - distilled water, ratio of raw materials and extractant - 1:50, stirring intensity - 500 rpm, temperature regime - 70 ⁰С, duration of extraction - 90 min. Velvet plants were grown in ecologically clean area of the Republic of Tatarstan, raw materials were collected in the flowering phase (August-September). Air-shade drying method was used for their preservation in order to ensure maximum possible preservation of antioxidants of phyto raw materials. To evaluate the stress tolerance index, the biotesting method was used on the biomodel of the equidisturbance infusoria Paramecium caudatum. Hydrogen peroxide and ethyl alcohol were used as stressors, characterising the antioxidant potential and membrane-stabilising properties of the studied phytoextracts, respectively.

Results. Based on a series of experimental studies, the effect of concentrations of the studied phytoextract in the range of 0.0076-0.435 mg/ml on biotest growth was determined. It was shown that all the studied concentrations of aqueous extract of Prostrate Velvet not only do not inhibit, but in some cases stimulate the growth of infusoria, which is especially characteristic of two- and three-day experiments. The optimum concentrations of stressors were found to be 1.5 per cent for hydrogen peroxide and 7 per cent for ethanol, respectively. It was revealed that the aqueous extract of inflorescences of velvet flowers increased stress resistance of the test subject, the most pronounced in relation to hydrogen peroxide.

Conclusion. As a result of a series of experimental studies, the optimal conditions for biotesting have been established when using as a test object the equidiferous infusoria Paramecium caudatum to evaluate the antioxidant potential of aqueous extracts of dried inflorescences of dried inflorescences of the ‘Carmen’ variety. The use of the following concentrations of stressors was proposed: 1.5% for hydrogen peroxide; 7% for ethanol. The time of immobilisation of infusoria during treatment with hydrogen peroxide and ethanol is 1±0.01 and 1.2±0.01 minutes, respectively. The concentrations of the studied phytoextract not inhibiting the test-object were determined. Their growth-stimulating effect on the biotest was shown due to the presence of protein and carbohydrate components in the studied object. It was revealed that aqueous extracts of inflorescences of Tagetes show antioxidant and membrane-stabilising effects in the concentration range of 0.03-0.435 mg/ml on dry matter of the extract.

EDN: GECDRZ