Manganese content in tea leaves (Camellia sinensis (L.) O. Kuntze) of the Colchis variety against the background of the use of various types of fertilizers in the subtropical conditions of the Russian Federation
Abstract
Background. The elemental composition of tea determines not only its food-flavoring properties, but is also a source of essential elements necessary for the normal functioning of our body, as in the modern world we often face a shortage of important elements as a result of an unbalanced diet.
A special place in tea is occupied by trace elements, which include iron (Fe), zinc (Zn), copper (Cu) and manganese (Mn). Tea plants accumulate large amounts of Mn in their leaves, which may be an important source of this trace element in the human diet. However, it should be noted that excessive consumption of tea can lead to excessive intake of manganese into the body.
For the first time in the subtropical zone of Russia, the interaction of manganese and the root application of a number of important trace elements (Mg, Ca, S, Zn, B) were studied, patterns of their influence on the manganese content in the leaves of the tea plant were identified, and the influence of meteorological factors on the accumulation of manganese was assessed.
Purpose. To determine the patterns of the intake of manganese compounds from the soil and its accumulation in the leaves of tea plants against the background of different mineral nutrition, from the point of view of food safety of agricultural products.
Materials and methods. The research was conducted in the area of the Black Sea coast of the Krasnodar Territory on a Colchis tea plantation. The experience includes 7 options: control and 6 options with trace elements. According to the experimental variants, mature leaves and juvenile shoots (3-leaf flush) were selected during the growing season in the period 2012-2023. The sample preparation of the leaves was carried out by the accelerated method of acid (wet) ozonization according to K.E. Ginzburg et al. (1963).
Results. It was found that the minimum manganese content in mature leaves was 1290 mg/kg, and the maximum was 3705 mg/kg, and a direct relationship was found between the manganese content and precipitation r=0.42. The manganese level in the 3-leaf flask ranged from 476 mg/kg in the first growth wave to 1246 mg/kg at the end of the flask collection period, and a direct correlation was established between the manganese content and the monthly average temperature r=0.56. It was revealed that the use of boron, despite the increase in yield, led to a decrease in the manganese content in juvenile shoots by 11.7–16.7% in July-August and by 23.6% in September compared with the control group.
Conclusion. The results show a complex relationship between manganese content, climatic conditions, and fertilizer application.
EDN: LEDGBH
Downloads
References
Агрохимические методы исследования почв / отв. ред. А. В. Соколов. (1975). Москва: Наука. 759 с.
Белоус, О. Г. (2006). Микроэлементы на чайных плантациях субтропиков России. Краснодар: КГАУ. 164 с. EDN: https://elibrary.ru/VUFXWN
Классификация и диагностика почв СССР / авт.-сост. В. В. Егоров, В. М. Фридланд, Е. И. Иванова и др. (1977). Москва: Колос. 223 с.
Малюкова, Л. С. (2011). Микроэлементы в системе почва — чайное растение в условиях субтропиков России. Сочи. 114 с. ISBN: 978 5 904533 13 7. EDN: https://elibrary.ru/UIDFDZ
Малюкова, Л. С., Притула, З. В., Козлова, Н. В., Керимзаде, В. В., & Великий, А. В. (2016). О формировании устойчивости у растений чая (Camellia sinensis (L.) O. Kuntze) при недостаточном водообеспечении на фоне корневого внесения кальция в виде природного удобрения. Сельскохозяйственная биология, 51(5), 673–679. https://doi.org/10.15389/agrobiology.2016.5.673eng. EDN: https://elibrary.ru/WZJQIT
Малюкова, Л. С., Цюпко, Т. Г., Притула, З. В., Воронова, О. Б., Гущаева, К. С., & Великий, А. В. (2018). Состав и содержание флавоноидов в готовом чае, выращенном в условиях Черноморского побережья Краснодарского края. В Сборник научных статей по материалам X Международного симпозиума «Фенольные соединения: функциональная роль в растениях» (№ 3, с. 272–277). Москва: PRESS-BOOK.RU. EDN: https://elibrary.ru/UVZCRL
Рындин, А. В., Малюкова, Л. С., Цюпко, Т. Г., Воронова, О. Б., & Гущаева, К. С. (2018). Особенности элементного состава Краснодарского чая сорта Колхида. Новые технологии, (4), 224–229. EDN: https://elibrary.ru/YXRFSX
Borse, B. B., Rao, L. J., Nagalakshmi, S., & Krishnamurthy, N. (2002). Fingerprint of black teas from India: Identification of the regio specific characteristics. Food Chemistry, 79, 419–424. https://doi.org/10.1016/S0308-8146(02)00191-7
Bouis, H. E., & Saltzman, A. (2017). Improving nutrition through biofortification: A review of evidence from Harvest Plus, 2003 through 2016. Global Food Security, 12, 49–58. https://doi.org/10.1016/j.gfs.2017.01.009
Diniz, P. H., Pistonesi, M. F., Alvarez, M. B., Band, B. F., & Araujo, M. C. (2015). Simplified tea classification based on a reduced chemical composition profile via successive projections algorithm linear discriminant analysis (SPA LDA). Journal of Food Composition and Analysis, 39, 103–110. https://doi.org/10.1016/j.jfca.2014.11.012
Ercisli, S., Orhan, E., Ozlem, S., Sengul, M., Gungor, N., & Orhan, E. (2008). Seasonal variation of total phenolic, antioxidant activity, plant nutritional elements, and fatty acids in tea leaves (Camellia sinensis var. sinensis clone Derepazari 7) grown in Turkey. Pharmaceutical Biology, 46, 10–11.
Malik, J., Szakova, J., Drabek, O., Balik, J., & Kokoska, L. (2008). Determination of certain micro and macroelements in plant stimulants and their infusions. Food Chemistry, 111(2), 520–525. https://doi.org/10.1016/j.foodchem.2008.04.009
Pang, Y., Abeysinghe, I. S., He, J., He, X., Huhman, D., Mewan, K. M., Sumner, L. W., Yun, J., & Dixon, R. A. (2013). Functional characterization of proanthocyanidin pathway enzymes from tea and their application for metabolic engineering. Plant Physiology, 161(3), 1103–1116. https://doi.org/10.1104/pp.112.212050. EDN: https://elibrary.ru/XZJXUM
Peng, J., Aved, A., Seetharaman, G., & Palaniappan, K. (2017). Multi view boosting with information propagation for classification. IEEE Transactions on Neural Networks and Learning Systems. https://doi.org/10.1109/TNNLS.2016.2637881
Samarina, L., Malyukova, L., Wang, S., Li, Y., Doroshkov, A., Bobrovskikh, A., et al. (2024). Nitrogen deficiency differentially affects lignin biosynthesis genes and flavanols accumulation in tolerant and susceptible tea genotypes (Camellia sinensis (L.) Kuntze). Plant Stress, 14, 100581. https://doi.org/10.1016/j.stress.2024.100581. EDN: https://elibrary.ru/TRQWKD
Shahzad, Z., & Amtmann, A. (2017). Food for thought: How nutrients regulate root system architecture. Current Opinion in Plant Biology, 39, 80–87. https://doi.org/10.1016/j.pbi.2017.06.008
Tseng, W., & Lai, H. (2022). Comprehensive analysis revealed the specific soil properties and foliar elements respond to the quality composition levels of tea (Camellia sinensis L.). Agronomy, 12(3), 670. https://doi.org/10.3390/agronomy12030670. EDN: https://elibrary.ru/WBOVIO
White, P. J., & Broadley, M. R. (2009). Biofortification of crops with seven mineral elements often lacking in human diets — Iron, Zinc, Copper, Calcium, Magnesium, Selenium and Iodine. New Phytologist, 182, 49–84. https://doi.org/10.1111/j.1469-8137.2008.02738. EDN: https://elibrary.ru/YCCVMX
Working Group WRB. (2014). World reference base for soil resources 2014: International soil classification system for naming soils and creating legends for soil maps. World Soil Resources Reports — FAO, (106). Rome. 181 с.
Xue, S. G., Chen, Y. X., Reeves, R. D., Baker, A. J. M., Lin, Q., & Fernando, D. R. (2004). Manganese uptake and accumulation by the hyperaccumulator plant Phytolacca acinosa Roxb. (Phytolaccaceae). Environmental Pollution, 131(3), 393–399. https://doi.org/10.1016/j.envpol.2004.03.011
Yashin, A. Y., Nemzer, B. V., Combet, E., & Yashin, Y. I. (2015). Determination of the chemical composition of tea by chromatographic methods: A review. Journal of Food Research, 4(3), 56–88. https://doi.org/10.5539/jfr.v4n3p56
Zaman, F., Zhang, E., Ihtisham, M., Ilyas, M., et al. (2023). Metabolic profiling, pigment component responses to foliar application of Fe, Zn, Cu, and Mn for tea plants (Camellia sinensis). Scientia Horticulturae, 319, 1. https://doi.org/10.1016/j.scienta.2023.112149. EDN: https://elibrary.ru/LOWPMV
Zhang, L., Zhang, J., Chen, L., Liu, T., Ma, G., & Liu, X. (2018). Influence of manufacturing process on the contents of iron, copper, chromium, nickel and manganese elements in Crush, Tear and Curl black tea, their transfer rates and health risk assessment. Food Control, 89. https://doi.org/10.1016/j.foodcont.2018.01.030
References
Agrochemical methods of soil research / ed. by A. V. Sokolov. (1975). Moscow: Nauka. 759 p.
Belous, O. G. (2006). Microelements in tea plantations of the subtropics of Russia. Krasnodar: Kuban State Agrarian University. 164 p. EDN: https://elibrary.ru/VUFXWN
Classification and diagnosis of soils of the USSR / comp. by V. V. Egorov, V. M. Fridland, E. I. Ivanova et al. (1977). Moscow: Kolos. 223 p.
Malyukova, L. S. (2011). Microelements in the soil tea plant system under the conditions of the Russian subtropics. Sochi. 114 p. ISBN: 978 5 904533 13 7. EDN: https://elibrary.ru/UIDFDZ
Malyukova, L. S., Pritula, Z. V., Kozlova, N. V., Kerimzade, V. V., & Velikiy, A. V. (2016). On the formation of resistance in tea plants (Camellia sinensis (L.) O. Kuntze) under insufficient water supply against the background of root application of calcium as a natural fertilizer. Agricultural Biology, 51(5), 673–679. https://doi.org/10.15389/agrobiology.2016.5.673eng. EDN: https://elibrary.ru/WZJQIT
Malyukova, L. S., Tsyupko, T. G., Pritula, Z. V., Voronova, O. B., Gushaeva, K. S., & Velikiy, A. V. (2018). Composition and content of flavonoids in finished tea grown under the conditions of the Black Sea coast of Krasnodar Krai. In Collection of scientific articles based on the materials of the X International Symposium “Phenolic compounds: functional role in plants” (No. 3, pp. 272–277). Moscow: PRESS-BOOK.RU. EDN: https://elibrary.ru/UVZCRL
Ryndin, A. V., Malyukova, L. S., Tsyupko, T. G., Voronova, O. B., & Gushaeva, K. S. (2018). Features of the elemental composition of Krasnodar tea of the Kolkhida variety. New Technologies, (4), 224–229. EDN: https://elibrary.ru/YXRFSX
Borse, B. B., Rao, L. J., Nagalakshmi, S., & Krishnamurthy, N. (2002). Fingerprint of black teas from India: Identification of the regio specific characteristics. Food Chemistry, 79, 419–424. https://doi.org/10.1016/S0308-8146(02)00191-7
Bouis, H. E., & Saltzman, A. (2017). Improving nutrition through biofortification: A review of evidence from Harvest Plus, 2003 through 2016. Global Food Security, 12, 49–58. https://doi.org/10.1016/j.gfs.2017.01.009
Diniz, P. H., Pistonesi, M. F., Alvarez, M. B., Band, B. F., & Araujo, M. C. (2015). Simplified tea classification based on a reduced chemical composition profile via successive projections algorithm linear discriminant analysis (SPA LDA). Journal of Food Composition and Analysis, 39, 103–110. https://doi.org/10.1016/j.jfca.2014.11.012
Ercisli, S., Orhan, E., Ozlem, S., Sengul, M., Gungor, N., & Orhan, E. (2008). Seasonal variation of total phenolic, antioxidant activity, plant nutritional elements, and fatty acids in tea leaves (Camellia sinensis var. sinensis clone Derepazari 7) grown in Turkey. Pharmaceutical Biology, 46, 10–11.
Malik, J., Szakova, J., Drabek, O., Balik, J., & Kokoska, L. (2008). Determination of certain micro and macroelements in plant stimulants and their infusions. Food Chemistry, 111(2), 520–525. https://doi.org/10.1016/j.foodchem.2008.04.009
Pang, Y., Abeysinghe, I. S., He, J., He, X., Huhman, D., Mewan, K. M., Sumner, L. W., Yun, J., & Dixon, R. A. (2013). Functional characterization of proanthocyanidin pathway enzymes from tea and their application for metabolic engineering. Plant Physiology, 161(3), 1103–1116. https://doi.org/10.1104/pp.112.212050. EDN: https://elibrary.ru/XZJXUM
Peng, J., Aved, A., Seetharaman, G., & Palaniappan, K. (2017). Multi view boosting with information propagation for classification. IEEE Transactions on Neural Networks and Learning Systems. https://doi.org/10.1109/TNNLS.2016.2637881
Samarina, L., Malyukova, L., Wang, S., Li, Y., Doroshkov, A., Bobrovskikh, A., et al. (2024). Nitrogen deficiency differentially affects lignin biosynthesis genes and flavanols accumulation in tolerant and susceptible tea genotypes (Camellia sinensis (L.) Kuntze). Plant Stress, 14, 100581. https://doi.org/10.1016/j.stress.2024.100581. EDN: https://elibrary.ru/TRQWKD
Shahzad, Z., & Amtmann, A. (2017). Food for thought: How nutrients regulate root system architecture. Current Opinion in Plant Biology, 39, 80–87. https://doi.org/10.1016/j.pbi.2017.06.008
Tseng, W., & Lai, H. (2022). Comprehensive analysis revealed the specific soil properties and foliar elements respond to the quality composition levels of tea (Camellia sinensis L.). Agronomy, 12(3), 670. https://doi.org/10.3390/agronomy12030670. EDN: https://elibrary.ru/WBOVIO
White, P. J., & Broadley, M. R. (2009). Biofortification of crops with seven mineral elements often lacking in human diets — Iron, Zinc, Copper, Calcium, Magnesium, Selenium and Iodine. New Phytologist, 182, 49–84. https://doi.org/10.1111/j.1469-8137.2008.02738. EDN: https://elibrary.ru/YCCVMX
Working Group WRB. (2014). World reference base for soil resources 2014: International soil classification system for naming soils and creating legends for soil maps. World Soil Resources Reports — FAO, (106). Rome. 181 с.
Xue, S. G., Chen, Y. X., Reeves, R. D., Baker, A. J. M., Lin, Q., & Fernando, D. R. (2004). Manganese uptake and accumulation by the hyperaccumulator plant Phytolacca acinosa Roxb. (Phytolaccaceae). Environmental Pollution, 131(3), 393–399. https://doi.org/10.1016/j.envpol.2004.03.011
Yashin, A. Y., Nemzer, B. V., Combet, E., & Yashin, Y. I. (2015). Determination of the chemical composition of tea by chromatographic methods: A review. Journal of Food Research, 4(3), 56–88. https://doi.org/10.5539/jfr.v4n3p56
Zaman, F., Zhang, E., Ihtisham, M., Ilyas, M., et al. (2023). Metabolic profiling, pigment component responses to foliar application of Fe, Zn, Cu, and Mn for tea plants (Camellia sinensis). Scientia Horticulturae, 319, 1. https://doi.org/10.1016/j.scienta.2023.112149. EDN: https://elibrary.ru/LOWPMV
Zhang, L., Zhang, J., Chen, L., Liu, T., Ma, G., & Liu, X. (2018). Influence of manufacturing process on the contents of iron, copper, chromium, nickel and manganese elements in Crush, Tear and Curl black tea, their transfer rates and health risk assessment. Food Control, 89. https://doi.org/10.1016/j.foodcont.2018.01.030
Copyright (c) 2025 Andrey V. Velikii
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
