Morphological changes and expression of caspases in cattle kidneys in age-related maner
Abstract
Background. Due to the high metabolic load, cow kidneys are particularly susceptible to industrial factors and undergo morphological changes, including cell death. Current literature lacks data on caspases in the kidneys of cattle of different age groups. This article presents morphological analysis and immunohistochemical staining results to detect caspase-3, -6, and -8 in the kidneys of cattle of different age groups.
Purpose. Evaluate morphological changes in the kidneys of cattle of different ages and their relationship with the expression of caspases.
Materials and methods. The study was conducted at the Ural Scientific Research Veterinary Institute – structural subdivision of Ural Federal Research Center of the Ural Branch of the Russian Academy of Sciences. To study morphological changes in the kidneys, postmortem specimens were collected from animals aged 2.5–10 months, 2–5 years, and 6–8 years. Immunohistochemical staining of the specimens with antibodies to caspases-3, -6, and -8 was performed to assess the intensity of apoptosis in the organ. The number and area of renal corpuscles, the area of the renal glomerulus and the space of the Bowman-Shumlyansky capsule, the proportion of area occupied by these cells within the renal corpuscle, and the proportion of damaged renal corpuscles were measured.
Results. The development of pathological processes in the kidneys of cattle begins at an early age. Morphometric analysis of kidney specimens revealed age-related pathological changes associated with high levels of caspase expression, particularly the effector caspases 3 and 6.
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References
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Copyright (c) 2027 Irina M. Petrova, Maksim V. Bytov, Ekaterina A. Tsepeleva, Sergey L. Khatsko, Lyudmila I. Drozdova, Irina A. Shkuratova
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