INFLUENCE OF DISINFECTANT ON CRYSTALLOGENIC ACTIVITY OF PSEUDOMONAS AERUGINOSA IN VITRO
Abstract
The purpose of this work was to clarify the crystallogenic properties of pseudomonads under the action of an antiseptic.
Material and methods. The material for the study was 8 strains of P. aeruginosa isolated from patients of the burn Department. In accordance with the purpose and objectives of the study, the work was performed in 3 stages: assessment of the biological properties of isolated pseudomonad strains; determination of sensitivity to disinfectants by the square method; assessment of the crystallogenic (initiating) activity of pseudomonads in individual and joint form with the disinfectant. The tested antiseptic was “Desam” in the form of a standard 1% solution used for disinfection of surfaces and medical instruments.
Results. It was found that all the studied Pseudomonas strains have the ability to activate the crystallogenesis of the basic substance (0.9% sodium chloride solution), which manifests itself both in qualitative and quantitative changes in the thesigraphic picture. It is shown that the addition of a common disinfectant (“Desam”) to the system “Pseudomonas aeruginosa – 0.9% sodium chloride solution” significantly transforms its dehydration structuring. At the same time, strains of the microorganism resistant to disinfectants moderately reduce the crystal’s gene activity (according to the main teziographic coefficient and belt coefficient). On the contrary, sensitive strains demonstrate a pronounced inhibition of the crystallogenesis of the basic substance. It allows to develop a new express method for determining the sensitivity of microorganisms to disinfectants.
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