EVALUATION OF THE EFFECT OF AUXINS ON GROWTH AND BIOCHEMICAL PARAMETERS OF CHAETOCEROS MUELLERI
Abstract
Background. The study of microalgae cultivation process is not only related to obtaining a wide range of biologically active substances. With the development of mariculture, some species of microalgae (including Chaetoceros muelleri) are of interest as food for mollusks, arthropods and holothuria. The aim of this study was to evaluate the effect of auxin hormones on quantitative and qualitative parameters Chaetoceros muelleri accumulative cultivation.
Materials and methods. The object of research was the culture of microalgae Chaetoceros muelleri. The duration of the experiment was 7 days. Indole-3- butyric and indole-3-acetic acids were used for growth stimulation. The microalgae were cultured in monoculture, under constant conditions. 0.1; 0.2; 0.4; 0.6; 1.0 ×10-5mol phytohormones concentrations were added to the cultivation medium of the experimental groups. The control group was cultured without phytohormones addition.
Results. It was shown that indole-3-acetic acid in concentrations from 0.2 to 0.5×10-5 mol had a positive effect on the growth of algal culture. Auxins at optimum concentration had a positive effect on lipid accumulation in the algal culture. It was found that indole-3-butyric acid increased the carbohydrate content in the culture on the 3rd day of culturing Chaetoceros muelleri. At the same time, under the action of indole-3-butyric acid, the highest content of carbohydrates.
Conclusion. The obtained data showed that the studied auxins had a positive effect on Chaetoceros muelleri dynamics growth. The possibility of using auxin series phytohormones to stimulate the growth of the microalga Chaetoceros muelleri in an accumulative monoculture has been established by this study.
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Список литературы
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Zhao Y., Wang H.P., Han B., Yu X. Coupling of abiotic stresses and phytohormones for the production of lipids and high-value by-products by microalgae: a review // Bioresource Technology. 2019. Vol. 274. P. 549-556.
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Bajguz A., Piotrowska-Niczyporuk A. Synergistic effect of auxins and brassinosteroids on the growth and regulation of metabolite content in the green alga Chlorella vulgaris (Trebouxiophyceae). Plant Physiology and Biochemistry, 2013, vol. 71, pp. 290-297.
Berard-Therriault L., Poulin M., Bosse L. Guide d'identification du phytoplancton marin de l'estuaire et du golfe du Saint-Laurent: incluant egalement certains protozoaires. Canadian Science Publishing (NRC Research Press) . 1999, 387 p.
Сarneiro M., Pojo V., Malcata F.X., Otero A. Lipid accumulation in selected Tetraselmis strains. Journal of Applied Phycology, 2019, vol. 31, no. 5, pp. 2845-2853.
Chen B., Wan C., Mehmood M.A., Chang J.S., Bai F., Zhao X. Manipulating environmental stresses and stress tolerance of microalgae for enhanced production of lipids and value-added products; a review. Bioresource Technology, 2017, vol. 244, pp. 1198-1206.
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Fazal T., Mushtaq A., Rehman F., Khan A.U., Rashid N., Farooq W., Xu J. Bioremediation of textile wastewater and successive biodiesel production using microalgae. Renewable & Sustainable Energy Reviews, 2018, vol. 82, pp. 3107-3126.
Gonzalez-Garcinu A., Sanchez-Alvarez J.A., Martin del Valle E.M. Understanding and optimizing the addition of phytohormones in the culture of microalgae for lipid production. Biotechnology Progress, 2016, vol. 32, no. 5, pp. 1203-1221.
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Hadizadeh Z., Mehrgan M.S., Shekarabi S.P.H. The potential use of stickwater from a kilka fishmeal plant in Dunaliella salina cultivation. Environmental Science and Pollution Research, 2020, vol. 27, pp. 2144-2154.
Han S.F., Jin W., Abomohra A.E.F., Zhou X., Tu R., Chen C., Wang Q. Enhancement of lipid production of Scenedesmus obliquus cultivated in municipal wastewater by plant growth regulator treatment. Waste Biomass Valorization, 2019, vol. 10, pp. 2479-2485.
Herbert D., Phipps P. J., Strange R. E. Chemical analysis of microbial cells. Methods in Microbiology, 1971, no. 5, pp. 209-344.
Johnson K.R., Ellis G., Toothill C. The sulfophosphovanilin reaction for serum lipids: a reappraisal. Clinical Chemistry, 1977, vol. 23, pp. 1669-1673.
Kawamura T., Roberts R.D., Nicholson C.M. Factors affecting the food value of diatom strains for post-larval abalone Haliotis iris. Aquaculture, 1998, vol. 160, pp. 81-88.
Kobraei M.E., White D.S. Effects of 2,4-dichlorophenoxyacetic acid on Kentucky Algae: simultaneous laboratory and field toxicity testings. Arch. Environ. Contam. Toxicol., 1996, vol. 31, pp. 571-580.
Koyande A.K., Chew K.W., Rambabu K., Tao Y., Chu D.T., Show P.L. Microalgae: A potential alternative to health supplementation for humans. Food Science and Human Wellness, 2019, vol. 8, pp. 16-24.
Kozlova T.A., Hardy B.P., Krishna P. & Levin D.B. Effect of phytohormones on growth and accumulation of pigments and fatty acids in the microalgae Scenedesmus quadricauda. Algal Research, 2017, vol. 27, pp. 325-334.
Laurens L.M.L., Dempster T.A., Jones H.D.T., Wolfrum E.J., Wychen S.V., McAllister J.S.P., Rencenberger M., Parchert K.J., Gloe L.M. Algal Biomass Constituent Analysis: Method Uncertainties and Investigation of the Underlying Measuring Chemistries. Journal of Analytical Chemistry, 2012, vol. 84, no. 4, pp. 1879-1887.
Lin B., Ahmed F., Du H., Li Z., Yan Y., Huang Y., Meng C. Plant growth regulators promote lipid and carotenoid accumulation in Chlorella vulgaris. Journal Applied Phycology, 2018, vol. 30, pp. 1549-1561.
Lowry O., Rosenbrougt N., Parr A., Randall R. Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry, 1951, vol. 193, no. 1, pp. 265-276.
Lu Y., Xu J. Phytohormones in microalgae: a new opportunity for microalgal biotechnology? Trends in Plant Science, 2015, vol. 20, pp. 272-283.
Measuring Growth Rates in Microalgal Cultures. A. M. Wood, R.C. Everroad, L.M. Wingard. Algal Culturing Technique [Andersen R.A.]. New York: Elsevier Academic Press, 2005, pp. 269-285.
Paliwal C., Mitra M., Bhayani K., Bharadwaj S.V., Ghosh T., Dubey S., Mishra S. Abiotic stresses as tools for metabolites in microalgae. Bioresource Technology, 2017, vol. 244, pp. 1216-1226.
Park W-K., Yoo G., Moon M., Kim C.W., Choi Y-E., Yang J-W. Phytohormone supplementation significantly increases growth of Chlamydomonas reinhardtii cultivated for biodiesel production. Appl. Biochem. Biotechnol., 2013, vol. 171, pp. 1128-1142.
Rastar M., Hosseini S.P., Shamsaie M.S., Sabzi S. Effects of iron and zinc concentrations on growth performance and biochemical composition of Haematococcus pluvialis: a comparison between nanoparticles and their corresponding metals bulks. Journal of Algal Biomass Utilization, 2018, vol. 9, pp. 59-67.
Renuka N., Guldhe A., Prasanna R., Singh P., Bux F. Microalgae as multi-functional options in modern agriculture: current trends, prospects and challenges. Biotechnology Advances, 2018, vol. 36, pp. 1255-1273.
Rines J.E.B., Theriot E.C. Systematics of Chaetocerotaceae (Bacillariophyceae): I. A phylogenetic analysis of the family. Physiol. Res., 2003, vol. 51, no. 2, pp. 83-98.
Rizwan M., Mujtaba G., Memon S.A., Lee K., Rashid N. Exploring the potential of microalgae for new biotechnology applications and beyond: a review. Renewable Sustainable Energy Reviews, 2018, vol. 92, pp. 394-404.
Sabzi S., Mehrgan M.S., Islami H.R., Shekarabi S.P.H. Changes in biochemical composition and fatty acid accumulation of Nannochloropsis oculata in response to different iron concentrations. Biofuels, 2018, vol. 12, pp. 1-7.
Salama E-S., Kabra A.N., Ji M-K., Kim J.R., Min B., Jeon B-H. Enhancement of microalgae growth and fatty acid content under the influence of phytohormones. Bioresource Technology, 2014, vol. 172, pp. 97-103.
Webb K., Chu F.L. Phytoplankton as a food source for algae. In: Pruder G.D., Langdon C.J., Conklin D.E. (eds). Proc. 2nd Int. Conf. Aquacult. Nutr. Spec. Publ. no 2. Louisiana State University, Baton Rouge, LA, 1983, pp. 272-291.
Zhao Y., Wang H.P., Han B., Yu X. Coupling of abiotic stresses and phytohormones for the production of lipids and high-value by-products by microalgae: a review. Bioresource Technology, 2019, vol. 274, pp. 549-556.
Zhuang L.L., Yu D., Zhang J., Liu F.F., Wu Y.H., Zhang T.Y., Hu H.Y. The characteristics and influencing factors of the attached microalgae cultivation: a review. Renewable and Sustainable Energy Reviews, 2018, vol. 94, pp. 1110-1119.
Zienkiewicz K., Du Z.Y., Ma W., Vollheyde K., Benning C. Stress-induced neutral lipid biosynthesis in microalgae-molecular, cellular and physiological insights. Biochimica et Biophysica Acta, 2016, vol. 1861, pp. 1269-1281.
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