PROBLEMS OF YEAR-ROUND MICROPROPIATION OF STRAWBERRY AND NEW APPROACHES TO THEIR SOLUTION
Abstract
Clonal micropropagation of strawberries can significantly accelerate the start of mass use of new highly productive cultivars. Mostly such material is needed for planting nurseries. However, the discrepancy between the annual working rhythm of the laboratory and the culture development cycle in natural conditions requires solving the problems of ensuring the viability of microplants during the period from late August to early March before planting in the greenhouse. At this time, it is often impossible to provide adequate conditions for the adaptation of microplants due to the low influx of sunlight and the high cost of using artificial sources.
Purpose.The purpose of the study was to develop a new scheme for working with test tube material obtained in the autumn-winter-spring period, which would eliminate the need for its adaptation immediately after the rooting of microcuttings.
Materials and methods. Strawberry cultivars of foreign selection Kimberly, Asia and Florence are studied. Cultivation was carried out on Murashige-Skoog medium at a temperature of 23±1°C, 16-hour photoperiod, illumination of 5-6 kLx. In microplants obtained within 1.5-2 months, after rooting, the formed roots and leaves were removed. Next, they were transplanted in the form of vegetating buds onto fresh medium containing auxin at a concentration of 0.1 mg L-1 for rehabilitation. Planting was carried out with 6-7 vegetating buds in 250 ml flasks.
Results. The restoration of leaves and roots of vegetating buds took place within 20-30 days, and their safety and survival rate was 100%. Subsequent repetitions of this procedure were also successful.
Conclusion.The new technique allows you to accurately plan the release of adapted strawberry microplants throughout the year. The material accumulated in the laboratory can be immediately adapted in a greenhouse with sufficient sunlight.
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Список литературы
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Karmaker S., Hossain Md.M., Hoque Md.A., Kaium Md.A., Ali T.B., Ferdous M. In Vitro Propagation of Three Strawberry Varieties and Field Evaluation. American Journal of Plant Sciences, 2023, vol. 14, pp. 1214-1222. https://doi.org/10.4236/ajps.2023.1411082
Karmaker S., Hossain Md.M., Hoque Md.A., Kaium Md.A., Al Amin Md., Islam Md.S. In Vitro Propagation of Three Strawberry Cultivars through Runner Tips Culture. American Journal of Plant Sciences, 2023, vol. 14, pp. 1296-1304. https://doi.org/10.4236/ajps.2023.1411087
Lal M., Sharma S., Hegde M.V. Micropropgation of Strawberry (Fragaria× ananassa Duch.). Indian Journal of Horticultural Research, 2003, vol. 37, pp. 231-234.
López-Aranda J.M., Soria C., Sanots B.M., Mirand L., Domínguez P., Medina-Mínguez J. Strawberry production in mild climates of the world: a review of current cultivar use. Intl. Journal of Fruit Sciences, 2011, vol. 11, pp. 232-224. https://doi.org/10.1080/15538362.2011.608294
Moradi K., Otroshy M., Azimi M.R. Micropropagation of strawberry by multiple shoots regeneration tissue cultures. Journal of Agricultural Technology, 2011, vol. 7, no. 6, pp. 1755-1763.
Munir M., Iqbal S., Baloch J.U.D., Khakwani A.A. In vitro explant sterilization and bud initiation studies of four strawberry cultivars. J. Appl. Hortic., 2015, vol. 17, no. 03, pp. 192–198. https://doi.org/10.37855/jah.2015.v17i03.36
Murashige T., Skoog F. A revised medium for rapid growth and bioassays with tobacco cultures. Physiol. Plant., 1962, vol. 15, pp. 473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Naing A.H., Kim S.H., Chun M.Y., Park S.K., Kim, C.K. In vitro propagation method for production of morphologically and genetically stable plants of different strawberry cultivars. Plant Methods, 2019, vol. 15, no. 1, pp. 36. https://doi.org/10.1186/s13007-019-0421-0
Park S.W., Kwack Y., Chun C. Growth of runner plants grown in a plant factory as affected by light intensity and container volume. Horticultural Science and Technology, 2017, vol. 35, no. 4, pp. 439-445. https://doi.org/10.12972/kjhst.20170047
Sabbadinia S., Marcellini M., Mezzetti B., Capocasa F. Establishing micropropagation protocols for new strawberry (Fragaria × ananassa) breeding lines. Acta Hortic., 2021, vol. 1309, pp. 573-578. https://doi.org/10.17660/ActaHortic.2021.1309.82
Sakila S., Ahmed M.B., Roy U.K., Biswas M.K., Karim R., Razvy M.A., Hossain M., Islam R., Hoque A. Micropropagation of Strawberry (Fragaria x ananassa Duch.) a Newly Introduced Crop in Bangladesh. American-Eurasian Journal of Scientific Research, 2007, vol. 2, pp. 151-154.
Steel R.G.D., Torrie J.H. Principle of statistics. A biometrical approach, 2nd. McGraw-Hill Kogakusha (Ltd), 1980, 245 p.
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