AGE CHANGES IN THE EXPRESSION LEVEL OF TIGHT JUNCTIONS MARKERS IN WOMEN AFTER MYOMECTOMY
Abstract
Background. Uterine fibroids are benign tumors that form from smooth muscle cells. Claudins (CLDNs) are tight junction proteins, their expression is different in tissues, the expression of CLDN is representative. CLDNs play an important role in neoplastic processes, as they are involved in the formation of a single signaling pathway between the extracellular matrix and the intracellular cytoskeleton.
Purpose. Objective of the study level of expression of markers responsible for the functional activity of CLDN1, CLDN7 and CLDN10 tight junctions in biopsy specimens of intact myometrium in women of different age groups.
Materials and methods. The study involved 90 patients aged 23 to 47 who were in randomly into 6 groups. The first three groups included 45 healthy patients, the fourth, fifth and sixth groups – women with uterine fibroids. The control groups and patients with uterine fibroids were divided into early, middle and late reproductive age.
The study was conducted with the approval of the Ethics Committee FSBSI “The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O.Ott” (Director – MD, professor Kogan I.Y.). Each participant signed an informed consent form for examination.
A complex of diagnostic techniques was carried out: anamnestic data, clinical and gynecological examination, echography, endoscopy, histological examination of scrapings and macropreparations removed during operations. Further, the material was studied using immunofluorescence analysis.
Statistical analysis was performed using the application «Excel 2010. Microsoft Office» and package “Statistica 10.0”.
Results. The results of the study show the level of expression of markers of tight intercellular contacts Claudin 1, Claudin 7 and Claudin 10 is reduced in the epithelium of cell membranes in women with uterine myoma.
Conclusion. A change in the functional activity of tight contacts leads to a disruption in the connections between cells. The expression level of claudins can be used as a marker and target for targeted therapy.
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References
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