ONTOGENETIC PROFILE OF THE GLUTATHIONE S-TRANSFERASE ACTIVITY IN MUSCA DOMESTICA L.
Abstract
Background. Musca domestica L. can transmit pathogens, and therefore poses serious threats to human and animal health. M. domestica is known as a model organism for studying the physiology and biochemistry of insects, the mechanisms of insecticidal resistance, as well as the development and testing of insecticides. The issue of insecticide resistance in insects is a concern all over the world; therefore, the study of insect enzymes is very important for a more complete understanding of the mechanisms underlying resistance to insecticides.
Purpose. To study the ontogenetic profile of glutathione-S-transferase activity in Musca domestica L.
Materials and methods. The glutathione-S-transferase activity was determined in M. domestica of laboratory (TY and UF) and field (Nov and Nik) strains at different ontogenetic stages. 1-Chloro-2,4-dinitrobenzene was used as the substrate and the optical density was recorded at 340 nm in kinetic mode. Specific activity was calculated taking into account the non-enzymatic transformation of the substrate, the dilution factor of the homogenate, and the protein content in the sample and was expressed as the change in optical density in 1 minute per mg of protein.
Results. The results obtained indicate changes in glutathione-S-transferase activity during the life cycle of M. domestica, both in laboratory and in field strains. Differences are observed between different stages of ontogenetic development. The enzyme activity in insects of the Lab TY strain was higher than that of the UF strain at almost all stages of the insect life cycle. For field strains, the GST activity was higher in specimens of the Nov strain compared to that of the Nik strain.
Conclusion. The differences of the GST activities in flies between two field strains may be due to differences in insecticidal load in the insect catching locations. The identified differences between flies of the laboratory and field strains may be associated with different genetic information. This may be related to the expression of certain enzyme isoforms, since the expression of enzymes can be influenced by various factors, including environmental factors, so that qualitatively different forms can be selected at the genetic level and differentially expressed. The results can be applied to the design of insect population management programs.
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Список литературы
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Larval Development and Molting / Kaleka A. S., Kaur N., Kour Bali G. Edible Insects, 2019. https://doi.org/10.5772/intechopen.85530
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Glutathione S–transferase in the defence against pyrethroids in insects / Kostaropoulos I., Papadopoulos A. I., Metaxakis A., Boukouvala E., Papadopoulou-Mourkidou E. // Insect Biochemistry and Molecular Biology, 2001, vol. 31, no. 4-5, pp. 313-319. https://doi.org/10.1016/s0965-1748(00)00123-5
Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. Protein measurement with Folin phenol reagent // Journal of Biological Chemistry, 1951, vol. 193, pp. 265-275. https://doi.org/10.1016/S0021-9258(19)52451-6
Masoud H. M. M., Helmy M. S., Darwish D. A., Ibrahim M. A. Purification, characterization, and enzyme kinetics of a glutathione S transferase from larvae of the camel tick Hyalomma dromedarii // Journal of Genetic Engineering and Biotechnology, 2023, vol. 21, no. 1, p. 28. https://doi.org/10.1186/s43141-023-00486-w
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Glutathione S-transferase in the developmental stages of the insect Apis mellifera macedonica / Papadopoulos A. I., Polemitou I., Laifi P., Yiangou A., Tananaki C. // Comparative Biochemistry and Physiology - Part C: Toxicology & Pharmacology, 2004, vol. 139, no. 1-3, pp. 87-92. https://doi.org/10.1016/j.cca.2004.09.009
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Shou-min, F. Insect glutathione S-transferase: a review of comparative genomic studies and response to xenobiotics // Bulletin of Insectology, 2012, vol. 65, pp. 265-271.
Insights into insecticide-resistance mechanisms in invasive species: Challenges and control strategies / Siddiqui J. А., Fan R., Naz H., Bamisile B. S., Hafeez M., Ghan M. I., Wei Y., Xu Y., Chen X. // Frontiers in physiology, 2023, vol. 13. https://doi:10.3389/fphys.2022.1112278
Silivanova E. A., Levchenko M. A. The activity of hydrolytic enzymes in different life stages of the house fly Musca domestica L. // Theory and practice of parasitic disease control, 2019, vol. 20, pp. 589-593. https://doi.org/10.31016/978-5-9902340-8-6.2019.20.589-593
Silivanova E. A., Levchenko M. A., Shumilova P. A., Plashkina V. A. Phosphatase and acetylcholinesterase activities in different stages of the life cycle of the housefly Musca domestica L. // Euroasian Entomological Journal, 2020, vol. 19, pp. 124-130. https://doi.org/10.15298/euroasentj.19.3.02
Wongtrakul J., Janphen K., Saisawang C., Ketterman A. J. Interaction of Omega, Sigma, and Theta glutathione transferases with p38b mitogen-activated protein kinase from the fruit fly, Drosophila melanogaster // Journal of Insect Science, 2014, vol. 14, no. 1, pp. 1-13. https://doi.org/10.1093/jis/14.1.60
Expression profiles of glutathione Stransferase superfamily in Spodoptera litura tolerated to sublethal doses of chlorpyrifos / Zhang N., Liu J., Chen S.-N., Huang L.-H., Feng Q.-L., Zheng S.-C. // Insect Science, 2016, vol. 23, pp. 675-687. https://doi.org/10.1111/1744-7917.12202
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Dmochowska-Ślęzak K., Giejdasz K., Fliszkiewicz M., Żółtowska K. Variations in antioxidant defense during the development of the solitary bee Osmia bicornis. Apidologie, 2015, vol. 46, no. 4, pp. 432-444. https://doi.org/10.1007/s13592-014-0333-y
Enayati A. A., Ranson H., Hemingway J. Insect glutathione transferases and insecticide resistance. Insect Molecular Biology, 2005, vol. 14, no. 1, pp. 3-8. https://doi.org/10.1111/j.1365-2583.2004.00529.x
Freeman J. C., Ross D. H., Scott J. G. Insecticide resistance monitoring of house fly populations from the United States. Pesticide Biochemistry and Physiology, 2019, vol. 158, pp. 61-68. https://doi.org/10.1016/j.pestbp.2019.04.006
Friedman R. Genomic organization of the glutathione S-transferase family in insects. Molecular Phylogenetics and Evolution, 2011, vol. 61, no. 3, pp. 924-932. https://doi.org/10.1016/j.ympev.2011.08.027
House Fly (Diptera: Muscidae): Biology, Pest Status, Current Management Prospects, and Research Needs / Geden C. J., Nayduch D., Scott J. G., Burgess E. R., IV, Gerry A. C., Kaufman P. E., Thomson J., Pickens V., Machtinger E. T. Journal of Integrated Pest Management, 2021, vol. 12, no. 1, pp. 1-38. https://doi.org/10.1093/jipm/pmaa021
Molecular Evolution of the Glutathione S-Transferase Family in the Bemisia tabaci Species Complex / Harari O. A., Santos-Garcia D., Musseri M., Moshitzky P., Patel M., Visendi P., Seal S., Sertchook R., Malka O., Morin S. Genome Biology and Evolution, 2020, vol. 12, no. 2, pp. 3857-3872. https://doi.org/10.1093/gbe/evaa002
Detection and functional characterization of sigma class GST in Phlebotomus argentipes and its role in stress tolerance and DDT resistance / Hassan F., Singh K. P., Ali V., Behera S., Shivam P., Das P., Dinesh D. S. Scientific Reports, 2019, vol. 9, pp. 1-15. https://doi.org/10.1038/s41598-019-56209-0
Larval Development and Molting / Kaleka A. S., Kaur N., Kour Bali G. Edible Insects, 2019. https://doi.org/10.5772/intechopen.85530
Kolawole A. O., Olajuyigbe F. M., Ajele J. O., Adedire C. O. Activity of the Antioxidant Defense System in a Typical Bioinsecticide-and Synthetic Insecticide-treated Cowpea Storage Beetle Callosobrochus maculatus F. (Coleoptera: Chrysomelidae). International Journal of Insect Science, 2014, vol. 6, pp. 99-108. https://doi.org/10.4137/IJIS.S19434
Kostaropoulos I., Mantzari A. E., Papadopoulos A. I. Alterations of some glutathione S-transferase characteristics during the development of Tenebrio molitor (Insecta: Coleoptera). Insect Biochemistry and Molecular Biology, 1996, vol. 26, no. 8-9, pp. 963-969. https://doi.org/10.1016/S0965-1748(96)00063-X
Glutathione S–transferase in the defence against pyrethroids in insects / Kostaropoulos I., Papadopoulos A. I., Metaxakis A., Boukouvala E., Papadopoulou-Mourkidou E. Insect Biochemistry and Molecular Biology, 2001, vol. 31, no. 4-5, pp. 313-319. https://doi.org/10.1016/s0965-1748(00)00123-5
Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. Protein measurement with Folin phenol reagent. Journal of Biological Chemistry, 1951, vol. 193, pp. 265-275. https://doi.org/10.1016/S0021-9258(19)52451-6
Masoud H. M. M., Helmy M. S., Darwish D. A., Ibrahim M. A. Purification, characterization, and enzyme kinetics of a glutathione S transferase from larvae of the camel tick Hyalomma dromedarii. Journal of Genetic Engineering and Biotechnology, 2023, vol. 21, no. 1, p. 28. https://doi.org/10.1186/s43141-023-00486-w
House Flies Are Underappreciated Yet Important Reservoirs and Vectors of Microbial Threats to Animal and Human Health / Nayduch D., Neupane S., Pickens V., Purvis T., Оlds C. Microorganisms, 2023, vol. 11, no. 3, p. 583. https://doi.org/10.3390/microorganisms11030583
Glutathione S-transferase in the developmental stages of the insect Apis mellifera macedonica / Papadopoulos A. I., Polemitou I., Laifi P., Yiangou A., Tananaki C. Comparative Biochemistry and Physiology - Part C: Toxicology & Pharmacology, 2004, vol. 139, no. 1-3, pp. 87-92. https://doi.org/10.1016/j.cca.2004.09.009
Pavlidi N., Vontas J., Van Leeuwen T. The role of glutathione S-transferases (GSTs) in insecticide resistance in crop pests and disease vectors. Current Opinion in Insect Science, 2018, vol. 27, pp. 97-102. https://doi.org/10.1016/j.cois.2018.04.007
Shou-min F. Insect glutathione S-transferase: a review of comparative genomic studies and response to xenobiotics. Bulletin of Insectology, 2012, vol. 65, pp. 265-271.
Insights into insecticide-resistance mechanisms in invasive species: Challenges and control strategies / Siddiqui J. А., Fan R., Naz H., Bamisile B. S., Hafeez M., Ghan M. I., Wei Y., Xu Y., Chen X. Frontiers in physiology, 2023, vol. 13. https://doi:10.3389/fphys.2022.1112278
Silivanova E. A., Levchenko M. A. The activity of hydrolytic enzymes in different life stages of the house fly Musca domestica L. Theory and practice of parasitic disease control, 2019, vol. 20, pp. 589-593. https://doi.org/10.31016/978-5-9902340-8-6.2019.20.589-593
Silivanova E. A., Levchenko M. A., Shumilova P. A., Plashkina V. A. Phosphatase and acetylcholinesterase activities in different stages of the life cycle of the housefly Musca domestica L. Euroasian Entomological Journal, 2020, vol. 19, pp. 124-130. https://doi.org/10.15298/euroasentj.19.3.02
Wongtrakul J., Janphen K., Saisawang C., Ketterman A. J. Interaction of Omega, Sigma, and Theta glutathione transferases with p38b mitogen-activated protein kinase from the fruit fly, Drosophila melanogaster. Journal of Insect Science, 2014, vol. 14, no. 1, pp. 1-13. https://doi.org/10.1093/jis/14.1.60
Expression profiles of glutathione Stransferase superfamily in Spodoptera litura tolerated to sublethal doses of chlorpyrifos / Zhang N., Liu J., Chen S.-N., Huang L.-H., Feng Q.-L., Zheng S.-C. Insect Science, 2016, vol. 23, pp. 675-687. https://doi.org/10.1111/1744-7917.12202
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