SIX-DAYS DYNAMICS ANXIETY INDEX OF FEMALE RATS WITH HIGH LEVEL OF ANXIETY IN BLOCKADE OF MAO-B
Abstract
Notoriously that motor and research activities are gradually oppressed up to complete suppression during the daily testing of animals. Generally, an animal stops researching the test space and demonstrates the passive behavior. In the past studies on female rats we have found out that the blockade of monoamine oxidase B (MAO-B) doesn’t prevent of the suppression of animal’s behavior activity during the daily testing. Also, according to the cumulative result it was supposed, that the increasing of anxiety can’t to be the main reason of motor deprivation. But the last statement required of experimental confirmation. According to the said statement the purpose of this work was formulated the following way: to find out the dependence of behavior activity oppression of female rats during the daily testing of anxiety level in normal conditions and in blockade of MAO-B one.
Methods: the research work was carried out on 48 white female rats line Wistar aged 7 months, contained in standard conditions of vivarium. All of the animals were homogenous sample with the middle level of motor activity and high level of anxiety (the index of anxiety (IA) were more than 0,7 of conventional units).
The rats were divided into four groups of 12 animal units in each one, lately -2 control sets and 2 experimental one (blockade of MAO-B). The animal behavioral reactions were researched in two tests: “Open field” (circular, diameter 1m) and elevated plus-maze (EPM, standard sized for rats). The residence time of each rat in both test setting was 5 minutes. This period of time is recorded by high definition camcorder; video recording is analyzed automatically by the program complex Noldus EthoVision 12.
It was found out, that the increasing the level anxiety cannot be the only reason of motor activity oppression of high-anxiety female rats in “Open field” and elevated plus-maze tests. The blockade of MAO-B decreases the index of anxiety of animals, that, possible, indicates on directly dopaminergic system and the central dopamine participation in the emotional state regulation along with other monoamines. Also the EPM test is more indicative for analyzing of dynamic of the anxiety index compared with “Open field” test.
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Список литературы
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