EFFECT OF FEED ADDITIVE DHQEC ON INDICATORS OF ANTIOXIDANT STATUS IN FATTENING PIGS
Abstract
World consumption of pork is steadily increasing, with the growing demand for pork being met by increasing both the number of pigs and their slaughter weight. However, pork production faces negative factors leading to the development of oxidative stress, which does not allow the genetic potential of the breeds to be fully realised. Particular attention is paid to the post-weaning period in pigs, when they are exposed to numerous stresses, with low adaptive capacity. This is an important period in pig rearing as it is often accompanied by temporary low feed intake, poor growth, intestinal dysbiosis and diarrhoea after weaning. These factors pose a threat to animal health and welfare, increasing the risk of mortality and leading to significant economic losses. For many years the problem of oxidative stress has been solved by the use of zinc oxide in the diets of weaned piglets. The use of natural antioxidant additives in pig diets is becoming more and more relevant in pig breeding practice. Natural antioxidants include ascorbic acid (vitamin C), tocopherol (vitamin E) and dihydroquercitin. The aim of our research was to create and test a feed complex with the additive properties of antioxidants and vitamins. We developed a new feed complex of Dihydroquercetin (DHQ) with vitamins E and C (DHQEC), which was fed to boars at a dose of 0.025% of the basic diet during the whole period of fattening. The concentration of reduced glutathione (GSH) in the blood serum of experimental animals was found to be significantly higher (p<0.1) by 12.5% at the end of the study compared with the control group. There was also a dynamics of oxidized glutathione concentration (GSS) decrease in experimental group animals, as well as a significant increase (p<0.05) of reduced to oxidized glutathione (GSH/GSS) ratio by 45,9%. The concentration of malonic dialdehyde (MDA) was reliably lower (P<0.05) by 33,6% in comparison with the control group, while the level of superoxide dismutase (SOD) activity was 48.2% higher in the period before slaughter (p<0,1) in experimental group. The main effect of feeding DHQEC in mixed fodders was evident in the improvement of average daily live weight gain (ADG), including at the beginning of the experiment, the 1st week (p<0.10), and in the first period of fattening (5-8 weeks, p=0.09; 8 weeks, p<0.05). These indices testify to effective action of antioxidants and promote reactivity of organism and stress resistance, which raises the indices of productivity.
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Варфоломеев С.Д., Пожитков А.Е. Активные центры гидролаз: основные типы структур и механизм катализа // Вестник Московского университета. Серия 2. Химия. 2000. Т. 41. № 3. С. 147-156.
Меерсон Ф.З., Пшенникова М.Г. Адаптация к стрессовым ситуациям и физическим нагрузкам. М.: Медицина,1988. 256 с.
Морозов В.И., Петрова Т.Н. Выявление протеиназ нейтрофилов в скелетных мышцах крыс после мышечной деятельности // Укр. биохим. журн. 1993. №65. С. 40-44.
Шенкман Б.С., Подлубная З.А., Вихлянцев И.М. Сократительные характеристики и белки саркомерного цитоскелета волокон m. soleus человека в условиях гравитационной разгрузки // Биофизика. 2004. № 49. С. 881-890.
Chung, Y.K., Mahan D.C., Lepine A.J. Efficacy of dietary D-α-tocopherol and DL-α-tocopheryl acetate for weanling pigs // J Anim Sci. 1992. №70. рр. 2485-2492.
Franco, R., Cidlowski J.A. Apoptosis and glutathione: beyond an antioxidant // Cell Death and Differentiation. 2009. №16. рр. 1303–1314.
Galano, A., Alvarez-Idaboy J.R. Glutathione: mechanism and kinetics of its non-enzymatic defense action against free radicals // RSC Advances. 2011. №1. рр. 1763–1771.
Håkansson, J. Hakkarainen J, Lundeheim N. Variation in vitamin E, glutathione perroxidase and retinol concentrations in blood plasma of primiparous sows and their piglets, and in vitamin E, selenium and retinol contents in sows' milk // Acta Agric Scand. Anim. Sci. 2001. №51. рр. 224-234.
Hedemann, M.S., Jensen S.K. Vitamin E status in newly weaned pigs is correlated to the activity of carboxyl ester hydrolase // In Proceeding of the 7th biennial conference of Australian Pig Science Association. 28. Nov. 1. Dec.1999, Adelaide Edited by: Cranwell P.D. Werribee, Victoria, Australian Pig Science Association; 1999:181.
Ishkaeva. R.A., Nizamov I.S., Blokhin D.S., Urakova E.A., Klochkov V.V., Nizamov I.D., Gareev B.I., Salakhieva D.V., Abdullin T.I. Dithiophosphate-Induced Redox Conversions of Reduced and Oxidized Glutathione // Molecules. 2021. №26. р. 2973. https://doi.org/10.3390/molecules26102973
Jacob, C., Battaglia E., Burkholz T., Peng D., Bagrel D., Montenarh M. Control of oxidative posttranslational cysteine modifications: from intricate chemistry tow ide spread biological and medical applications // Chemical Research in Toxicology. 2012. №25. рр. 588–604.
Leskovec, J., Rezar V., Svete A.N., Salobir J., Levart A. Antioxidative Effects of Olive Polyphenols Compared to Vitamin E in Piglets Fed a Diet Rich in N-3 PUFA // Animals. 2019. №12. https://doi.org/10.3390/ani9040161
Liu, T., Sun L., Zhang Y., Wang Y., Zheng J. Imbalanced GSH/ROS and sequential cell death // J Biochem. Mol. Toxicol. 2022. №36. https://doi.org/10.1002/jbt.22942
Lv, H., Zhen C., Liu J., Yang P., Hu L., Shang P. Unraveling the Potential Role of Glutathione in Multiple Forms of Cell Death in Cancer Therapy // Oxid Med Cell Longev. 2019. 3150145. https://doi.org/10.1155/2019/3150145
Meyer, W.R., Mahan D.C., Moxon A.L. Value of dietary selenium and vitamin E for weanling swine as measured by performance and tissue selenium and glutathione peroxidase activities // J Anim. Sci. 1981. №52. рр. 302-311.
Moreira I., Mahan D.C. Effect of dietary levels of vitamin E (all-rac-tocopheryl acetate) with or without added fat on weanling pig performance and tissue alpha-tocopherol concentration // J. Anim. Sci. 2002. № 80. рр. 663-665.
Moreira I., Mahan D.C. Effect of dietary levels of vitamin E (all-rac-tocopheryl acetate) with or without added fat on weanling pig performance and tissue alpha-tocopherol concentration // J. Anim. Sci. 2002. № 80. рр. 665-669.
Nagy, P. Kinetics and mechanisms of thiol-disulfide exchange covering direct substitution and thiol oxidation-mediated pathways. // Antioxidants & Redox Signaling. 2013. №18. рр. 1623–1641.
Нормы потребностей молочного скота и свиней в питательных веществах: Монография /Под ред. Р.В. Некрасова, А.В. Головина, Е.А. Махаева / Некрасов Р.В., Головин А.В., Махаев Е.А., Аникин А.С., Первов Н.Г., Стрекозов Н.И., Мысик А.Т., Дуборезов В.М., Чабаев М.Г., Фомичев Ю.П., Гусев И.В. М., 2018. 290 с.
Ogata, K., Imai A., Sato S., Nishino K., Watanabe S., Somfai T., Kobayashi E., Takeda K. Effects of reduced glutathione supplementation in semen freezing extender on frozen-thawed bull semen and in vitro fertilization // J. Reprod. Dev. 2022. №18. рр. 53-61. https://doi.org/10.1262/jrd.2021-079
Owen, J.B., Butterfield D.A. Measurement of oxidized/reduced glutathione ratio // Methods Mol Biol. 2010. №648. рр. 269-77. https://doi.org/10.1007/978-1-60761-756-3_18
Shimura, T., Nakashiro C., Fujiwara K., Shiga R., Sasatani M., Kamiya K., Ushiyama A. Radiation affects glutathione redox reaction by reduced glutathione peroxidase activity in human fibroblasts // J Radiat Res. 2022. №17. рр. 183-191. https://doi.org/10.1093/jrr/rrab122
Sivertsen, T., Vie E., Bernhoft A., Baustad B. Vitamin E and selenium plasma concentrations in weanling pigs under field conditions in Norwegian pig herds // Acta Vet Scand. 2007. №49. https://doi.org/10.1186/1751-0147-49-1
Stuart, R.L., Kane E. Vitamin E form, source may be important for swine // Feedstuffs. 2004. №76. рр. 11-14.
Teige, J., Tollersrud S., Lund A., Larsen H.J. Swine dysentery: the influence of dietary vitamin E and selenium on the clinical and pathological effects of Treponemahyodysenteriae infection in pigs // Res Vet Sci. 1982. №32. рр. 95-100.
Van Kempen, T.A., Reijersen M.H., de Bruijn C., De Smet S., Michiels J., Traber M.G., Lauridsen C. Vitamin E plasma kinetics in swine show low bioavailability and short half-life of -α-tocopheryl acetate // J Anim Sci. 2016. №94. рр. 4188-4195. https://doi.org/10.2527/jas.2016-0640
Winterbourn, C.C. Superoxideas an intracellular radical sink // Free Radical Biology and Medicine. 1993. №14. рр. 85–90.
Wuryastuti, H., Stowe H.D., Bull R.W., Miller E.R. Effects of vitamin E and selenium on immune responses of peripheral blood, colostrum, and milk leukocytes of sows // J AnimSci. 1993. №71. рр. 2464-2472.
References
Varfolomeev S.D., Pozhitkov A.E. Vestnik Moskovskogo universiteta. Seriya 2. Khimiya, 2000, vol. 41, no. 3, pp. 147-156.
Meerson F.Z., Pshennikova M.G. Adaptatsiya k stressovym situatsiyam i fizicheskim nagruzkam [Adaptation to stressful situations and physical loads]. M.: Meditsina, 1988, 256 p.
Morozov V.I., Petrova T.N. Ukr. biokhim. zhurn., 1993, no. 65, pp. 40-44.
Shenkman B.S., Podlubnaya Z.A., Vikhlyantsev I.M. Biofizika, 2004, no. 49, pp. 881-890.
Chung, Y.K., Mahan D.C., Lepine A.J. Efficacy of dietary D-α-tocopherol and DL-α-tocopheryl acetate for weanling pigs. J Anim Sci., 1992, no. 70, pp. 2485-2492.
Franco, R., Cidlowski J.A. Apoptosis and glutathione: beyond an antioxidant. Cell Death and Differentiation, 2009, no. 16, pp. 1303–1314.
Galano, A., Alvarez-Idaboy J.R. Glutathione: mechanism and kinetics of its non-enzymatic defense action against free radicals. RSC Advances, 2011, no. 1, pp. 1763–1771.
Håkansson, J. Hakkarainen J, Lundeheim N. Variation in vitamin E, glutathione perroxidase and retinol concentrations in blood plasma of primiparous sows and their piglets, and in vitamin E, selenium and retinol contents in sows' milk. Acta Agric Scand. Anim. Sci., 2001, no. 51, pp. 224-234.
Hedemann, M.S., Jensen S.K. Vitamin E status in newly weaned pigs is correlated to the activity of carboxyl ester hydrolase. Proceeding of the 7th biennial conference of Australian Pig Science Association. 28. Nov. 1. Dec. 1999, Adelaide Edited by: Cranwell P.D. Werribee, Victoria, Australian Pig Science Association; 1999:181.
Ishkaeva. R.A., Nizamov I.S., Blokhin D.S., Urakova E.A., Klochkov V.V., Nizamov I.D., Gareev B.I., Salakhieva D.V., Abdullin T.I. Dithiophosphate-Induced Redox Conversions of Reduced and Oxidized Glutathione. Molecules, 2021, no. 26, p. 2973. https://doi.org/10.3390/molecules26102973
Jacob, C., Battaglia E., Burkholz T., Peng D., Bagrel D., Montenarh M. Control of oxidative posttranslational cysteine modifications: from intricate chemistry tow ide spread biological and medical applications. Chemical Research in Toxicology, 2012, no. 25, pp. 588–604.
Leskovec, J., Rezar V., Svete A.N., Salobir J., Levart A. Antioxidative Effects of Olive Polyphenols Compared to Vitamin E in Piglets Fed a Diet Rich in N-3 PUFA. Animals, 2019, no. 12. https://doi.org/10.3390/ani9040161
Liu, T., Sun L., Zhang Y., Wang Y., Zheng J. Imbalanced GSH/ROS and sequential cell death. J Biochem. Mol. Toxicol., 2022, no. 36. https://doi.org/10.1002/jbt.22942
Lv, H., Zhen C., Liu J., Yang P., Hu L., Shang P. Unraveling the Potential Role of Glutathione in Multiple Forms of Cell Death in Cancer Therapy. Oxid Med Cell Longev., 2019, 3150145. https://doi.org/10.1155/2019/3150145
Meyer, W.R., Mahan D.C., Moxon A.L. Value of dietary selenium and vitamin E for weanling swine as measured by performance and tissue selenium and glutathione peroxidase activities. J Anim. Sci., 1981, no. 52, pp. 302-311.
Moreira I., Mahan D.C. Effect of dietary levels of vitamin E (all-rac-tocopheryl acetate) with or without added fat on weanling pig performance and tissue alpha-tocopherol concentration. J. Anim. Sci., 2002, no. 80, pp. 663-665.
Moreira I., Mahan D.C. Effect of dietary levels of vitamin E (all-rac-tocopheryl acetate) with or without added fat on weanling pig performance and tissue alpha-tocopherol concentration. J. Anim. Sci., 2002, no. 80, pp. 665-669.
Nagy, P. Kinetics and mechanisms of thiol-disulfide exchange covering direct substitution and thiol oxidation-mediated pathways. Antioxidants & Redox Signaling, 2013, no. 18, pp. 1623–1641.
Normy potrebnostey molochnogo skota i sviney v pitatel'nykh veshchestvakh: Monografiya [Norms of dairy cattle and pigs nutrient requirements: Monograph] /Pod red. R.V. Nekrasova, A.V. Golovina, E.A. Makhaeva / Nekrasov R.V., Golovin A.V., Makhaev E.A., Anikin A.S., Pervov N.G., Strekozov N.I., Mysik A.T., Duborezov V.M., Chabaev M.G., Fomichev Yu.P., Gusev I.V. M., 2018, 290 p.
Ogata, K., Imai A., Sato S., Nishino K., Watanabe S., Somfai T., Kobayashi E., Takeda K. Effects of reduced glutathione supplementation in semen freezing extender on frozen-thawed bull semen and in vitro fertilization. J. Reprod. Dev., 2022, no. 18, pp. 53-61. https://doi.org/10.1262/jrd.2021-079
Owen, J.B., Butterfield D.A. Measurement of oxidized/reduced glutathione ratio. Methods Mol Biol., 2010, no. 648, pp. 269-77. https://doi.org/10.1007/978-1-60761-756-3_18
Shimura, T., Nakashiro C., Fujiwara K., Shiga R., Sasatani M., Kamiya K., Ushiyama A. Radiation affects glutathione redox reaction by reduced glutathione peroxidase activity in human fibroblasts. J Radiat Res., 2022, no. 17, pp. 183-191. https://doi.org/10.1093/jrr/rrab122
Sivertsen, T., Vie E., Bernhoft A., Baustad B. Vitamin E and selenium plasma concentrations in weanling pigs under field conditions in Norwegian pig herds. Acta Vet Scand., 2007, no. 49. https://doi.org/10.1186/1751-0147-49-1
Stuart, R.L., Kane E. Vitamin E form, source may be important for swine. Feedstuffs, 2004, no. 76, pp. 11-14.
Teige, J., Tollersrud S., Lund A., Larsen H.J. Swine dysentery: the influence of dietary vitamin E and selenium on the clinical and pathological effects of Treponemahyodysenteriae infection in pigs. Res Vet Sci., 1982, no. 32, pp. 95-100.
Van Kempen, T.A., Reijersen M.H., de Bruijn C., De Smet S., Michiels J., Traber M.G., Lauridsen C. Vitamin E plasma kinetics in swine show low bioavailability and short half-life of -α-tocopheryl acetate. J Anim Sci., 2016, no. 94, pp. 4188-4195. https://doi.org/10.2527/jas.2016-0640
Winterbourn, C.C. Superoxideas an intracellular radical sink. Free Radical Biology and Medicine, 1993, no. 14, pp. 85–90.
Wuryastuti, H., Stowe H.D., Bull R.W., Miller E.R. Effects of vitamin E and selenium on immune responses of peripheral blood, colostrum, and milk leukocytes of sows. J AnimSci., 1993, no. 71, pp. 2464-2472.
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